@article{fdi:010073981,
  title = {{C}omplexin in ivermectin resistance in body lice},
  author = {{A}manzougaghene, {N}. and {F}enollar, {F}. and {N}appez, {C}. and {B}en-{A}mara, {A}. and {D}ecloquement, {P}. and {A}zza, {S}. and {B}echah, {Y}. and {C}habriere, {E}. and {R}aoult, {D}. and {M}ediannikov, {O}leg},
  editor = {},
  language = {{ENG}},
  abstract = {{I}vermectin has emerged as very promising pediculicide, particularly in cases of resistance to commonly used pediculicides. {R}ecently, however, the first field-evolved ivermectin-resistance in lice was reported. {T}o gain insight into the mechanisms underlying ivermectin-resistance, we both looked for mutations in the ivermectin-target site ({G}lu{C}l) and searched the entire proteome for potential new loci involved in resistance from laboratory susceptible and ivermectin-selected resistant body lice. {P}olymorphism analysis of c{DNA} {G}lu{C}l showed no non-silent mutations. {P}roteomic analysis identified 22 differentially regulated proteins, of which 13 were upregulated and 9 were downregulated in the resistant strain. {W}e evaluated the correlation between m{RNA} and protein levels by q{RT}-{PCR} and found that the trend in transcriptional variation was consistent with the proteomic changes. {A}mong differentially expressed proteins, a complexin i.e. a neuronal protein which plays a key role in regulating neurotransmitter release, was shown to be the most significantly down-expressed in the ivermectin-resistant lice. {M}oreover, {DNA}-mutation analysis revealed that some complexin transcripts from resistant lice gained a premature stop codon, suggesting that this down-expression might be due, in part, to secondary effects of a nonsense mutation inside the gene. {W}e further confirmed the association between complexin and ivermectin-resistance by {RNA}-interfering and found that knocking down the complexin expression induces resistance to ivermectin in susceptible lice. {O}ur results provide evidence that complexin plays a significant role in regulating ivermectin resistance in body lice and represents the first evidence that links complexin to insecticide resistance.},
  keywords = {},
  booktitle = {},
  journal = {{PL}o{S} {G}enetics},
  volume = {14},
  numero = {8},
  pages = {e1007569 [18 p.]},
  ISSN = {1553-7404},
  year = {2018},
  DOI = {10.1371/journal.pgen.1007569},
  URL = {https://www.documentation.ird.fr/hor/fdi:010073981},
}