@article{fdi:010073864,
  title = {{N}o evidence of interspecific genetic exchange by multi-locus microsatellite typing between {L}eishmania killicki and {L}eishmania major in a mixed focus of cutaneous {L}eishmaniasis in {S}outheast {T}unisia},
  author = {{H}arrabi, {M}. and {G}hawar, {W}. and {H}ide, {M}. and {B}attaieb, {J}. and {Y}azidi, {R}. and {J}aouadi, {K}. and {S}ana, {C}. and {C}halga, {B}. and {S}ana, {C}. and {C}halga, {B}. and {T}oumi, {A}. and {Z}aâtour, {A}. and {R}ou{\`e}ne, {M}. and {B}añuls, {A}nne-{L}aure and {B}en {S}alah, {A}.},
  editor = {},
  language = {{ENG}},
  abstract = {{S}ixty-four {L}eishmania samples were isolated from patients in several villages in the {T}ataouine governorate, southeast {T}unisia. {T}his region is known to be a mixed focus of human cutaneous {L}eishmaniasis caused by {L}eishmania ({L}.) killicki (synonymous {L}. tropica) and {L}. major. {T}o identify the {L}eishmania species in this governorate, a nested polymerase chain reaction based on the variable region of the kinetoplast minicircle was performed on each isolate. {M}ulti-locus microsatellite typing using markers selected for their ability to amplify the two species was used to explore patterns of interspecific genetic exchange. {T}hirteen {L}. major and 51 {L}. killicki isolates were identified. {T}he analysis of microsatellite data showed very low genetic diversity in each species with this set of microsatellites but a high differentiation between the two species. {N}ine {L}. major and five {L}. killicki strains revealed heterozygous genotypes with no shared allele between the two species. {T}hese heterozygotes probably resulted from genetic mutation events and not from interspecific genetic exchange. {S}pecific and different epidemiological cycles at the sympatric level might explain the absence of genetic exchange between the two {L}eishmania species in the {T}ataouine governorate.},
  keywords = {{LEISHMANIOSE} ; {EPIDEMIOLOGIE} ; {PARASITE} ; {GENOTYPE} ; {HETEROZYGOTE} ; {BIOLOGIE} {MOLECULAIRE} ; {MUTATION} ; {MARQUEUR} {MICROSATELLITE} ; {TUNISIE} ; {TATAOUINE}},
  booktitle = {},
  journal = {{J}ournal of {I}nfectious {D}iseases and {P}reventive {M}edicine},
  volume = {5},
  numero = {2},
  pages = {art. no 10000163 [7 ]},
  ISSN = {2329-8731},
  year = {2017},
  URL = {https://www.documentation.ird.fr/hor/fdi:010073864},
}