@article{fdi:010073846,
  title = {{C}oxiella burnetii transcriptional analysis reveals serendipity clusters of regulation in intracellular bacteria},
  author = {{L}eroy, {Q}. and {L}ebrigand, {K}. and {A}rmougom, {F}abrice and {B}arbry, {P}. and {T}hiery, {R}. and {R}aoult, {D}idier},
  editor = {},
  language = {{ENG}},
  abstract = {{C}oxiella burnetii, the causative agent of the zoonotic disease {Q} fever, is mainly transmitted to humans through an aerosol route. {A} spore-like form allows {C}. burnetii to resist different environmental conditions. {B}ecause of this, analysis of the survival strategies used by this bacterium to adapt to new environmental conditions is critical for our understanding of {C}. burnetii pathogenicity. {H}ere, we report the early transcriptional response of {C}. burnetii under temperature stresses. {O}ur data show that {C}. burnetii exhibited minor changes in gene regulation under short exposure to heat or cold shock. {W}hile small differences were observed, {C}. burnetii seemed to respond similarly to cold and heat shock. {T}he expression profiles obtained using microarrays produced in-house were confirmed by quantitative {RT}-{PCR}. {U}nder temperature stresses, 190 genes were differentially expressed in at least one condition, with a fold change of up to 4. {G}lobally, the differentially expressed genes in {C}. burnetii were associated with bacterial division, (p) pp{G}pp synthesis, wall and membrane biogenesis and, especially, lipopolysaccharide and peptidoglycan synthesis. {T}hese findings could be associated with growth arrest and witnessed transformation of the bacteria to a spore-like form. {U}nexpectedly, clusters of neighboring genes were differentially expressed. {T}hese clusters do not belong to operons or genetic networks; they have no evident associated functions and are not under the control of the same promoters. {W}e also found undescribed but comparable clusters of regulation in previously reported transcriptomic analyses of intracellular bacteria, including {R}ickettsia sp. and {L}isteria monocytogenes. {T}he transcriptomic patterns of {C}. burnetii observed under temperature stresses permits the recognition of unpredicted clusters of regulation for which the trigger mechanism remains unidentified but which may be the result of a new mechanism of epigenetic regulation.},
  keywords = {},
  booktitle = {},
  journal = {{P}los {O}ne},
  volume = {5},
  numero = {12},
  pages = {e15321},
  ISSN = {1932-6203},
  year = {2010},
  DOI = {10.1371/journal.pone.0015321},
  URL = {https://www.documentation.ird.fr/hor/fdi:010073846},
}