@article{fdi:010073803,
  title = {{I}nterdependency of efficient nodulation and arbuscular mycorrhization in {P}iptadenia gonoacantha, a {B}razilian legume tree},
  author = {{B}ournaud, {C}. and {J}ames, {E}. {K}. and de {F}aria, {S}. {M}. and {L}ebrun, {M}ichel and {M}elkonian, {R}. and {D}uponnois, {R}obin and {T}isseyre, {P}ierre and {M}oulin, {L}ionel and {P}rin, {Y}.},
  editor = {},
  language = {{ENG}},
  abstract = {{T}ripartite interactions between legumes and their root symbionts (rhizobia and arbuscular mycorrhizal fungi, {AMF}) are poorly understood, although it is well established that only specific combinations of symbionts lead to optimal plant growth. {A} classic example in which to investigate such interactions is the {B}razilian legume tree {P}iptadenia gonoacantha ({C}aesalpinioideae), for which efficient nodulation has been described as dependent on the presence of {AMF} symbiosis. {I}n this study, we compared the nodulation behaviour of several rhizobial strains with or without {AMF} inoculation, and performed analyses on nodulation, nodule cytology, {N}-fixing efficiency, and plant growth response. {N}odulation of {P}.gonoacantha does not rely on the presence of {AMF}, but mycorrhization was rhizobial strain-dependent, and nodule effectiveness and plant growth were dependent on the presence of specific combinations of rhizobial strains and {AMF}. {T}he co-occurrence of both symbionts within efficient nodules and the differentiation of bacteroids within nodule cells were also demonstrated. {N}ovel close interactions and interdependency for the establishment and/or functioning of these symbioses were also revealed in {P}iptadenia, thanks to immunocytochemical analyses. {T}hese data are discussed in terms of the evolutionary position of the newly circumscribed mimosoid clade within the {C}aesalpinioid subfamily and its relative proximity to non-nodulated (but {AMF}-associated) basal subfamilies. {T}he context of this work is based on the observation that among the {C}aesalpinioideae, in a basal position of the legume phylogeny, are grouped together, nodulated and non-nodulated clades of plants. {A}ll these plants are considered to be arbuscular mycorrhizal ({AM}), so we focused our attention on a group that includes the genus {P}iptadenia for which nodulation efficiency has been previously suggested to be directly linked to the presence of the {AM} symbiosis, taking into consideration the evolutionary relationship between both {AM} and rhizobial symbioses. {O}n the nodulated tree {P}iptadenia gonoacantha, our hypothesis was that for optimum efficiency both nitrogen-fixing and {AM} symbioses have to share a common niche to closely interact with each other, that is, the nodule. {W}e showed that both symbioses not only coexist, but that they interdependently exist, with both microsymbionts interacting to specifically promote the establishment or functioning of the other, and then leading to improved growth of the host plant.},
  keywords = {arbuscular mycorrhizal fungi ; {B}razil ; nitrogen fixation ; {P}araburkholderia ; {P}iptadenia gonoacantha ; rhizobia ; tripartite symbiosis ; {BRESIL}},
  booktitle = {},
  journal = {{P}lant {C}ell and {E}nvironment},
  volume = {41},
  numero = {9},
  pages = {2008--2020},
  ISSN = {0140-7791},
  year = {2018},
  DOI = {10.1111/pce.13095},
  URL = {https://www.documentation.ird.fr/hor/fdi:010073803},
}