@article{fdi:010073792,
  title = {{I}mmunodetection and molecular determination of visceral and cutaneous {L}eishmania infection using patients' urine},
  author = {{M}irzaei, {A}. and {A}hmadipour, {F}. and {C}annet, {A}. and {M}arty, {P}. and {D}elaunay, {P}. and {P}errin, {P}. and {D}orkeld, {F}. and {S}ereno, {D}enis and {A}khoundi, {M}.},
  editor = {},
  language = {{ENG}},
  abstract = {{T}he diagnosis of leishmaniasis relies mainly on the use of invasive processes, to collect the biological material for detecting {L}eishmania parasites. {B}ody fluids, which can be collected by non-invasive process, would greatly facilitate the leishmaniasis diagnosis. {I}n the present study, we investigated the potency of urine immunoblotting to diagnose cutaneous and visceral leishmaniasis and we compared with routine molecular methods. {A} total of 80 samples, including 40 sera and their 40 corresponding urine samples were collected from 37 suspected patients with cutaneous and visceral leishmaniasis, and 3 healthy individuals (as control), in {I}lam and {A}rdabil provinces of {I}ran. {A}ll sera and urine samples were analyzed, using immunoblotting. {T}he confirmation of leishmaniasis infection was performed, using conventional and quantitative {PCR}s as well as by sequencing the amplicons. {A}mong 37 suspected patients, 23 patients presented cutaneous lesions ({CL}) and 14 exhibited clinical symptoms reminiscent of visceral leishmaniasis ({L}. infantum). {A}mong cutaneous patients, 15 were positive for zoonotic cutaneous leishmaniasis ({L}. major), and eight for anthroponotic cutaneous leishmaniasis ({L}. tropica). {M}olecular quantification of {L}eishmania parasites was performed on sera, urines and cutaneous biopsies of {CL} and {VL} patients, demonstrating that parasite load is lower in urines, compared to sera or biopsy. {DNA} can be detected in 20 out of 23 (86.9%) {CL} urine samples and in 13 out of 14 (92.8%) {VL} urine samples. {I}mmunodetection analysis demonstrates that 22 out of 23 (95.6%) sera from {CL} patients and all patients suspected with {VL} are positive. {F}or urine samples, 18 out of 23 (78.2%) urine of {CL} patients and 13 out of 14 (92.8%) urine of {VL} patients were positive, using {W}estern blot. {T}herefore, immunodetection and molecular analysis using urine samples can be used as a diagnostic tool for surveying cutaneous and visceral leishmaniasis.},
  keywords = {{L}eishmaniasis ; {W}estern blot ; q{PCR} ; {S}erum ; {U}rine ; {IRAN}},
  booktitle = {},
  journal = {{I}nfection {G}enetics and {E}volution},
  volume = {63},
  numero = {},
  pages = {257--268},
  ISSN = {1567-1348},
  year = {2018},
  DOI = {10.1016/j.meegid.2018.05.021},
  URL = {https://www.documentation.ird.fr/hor/fdi:010073792},
}