@article{fdi:010073769,
  title = {{CRISPR}/{C}as9-mediated efficient targeted mutagenesis has the potential to accelerate the domestication of {C}offea canephora},
  author = {{B}reitler, {J}. {C}. and {D}echamp, {E}. and {C}ampa, {C}laudine and {R}odrigues, {L}. {A}. {Z}. and {G}uyot, {R}omain and {M}arraccini, {P}. and {E}tienne, {H}.},
  editor = {},
  language = {{ENG}},
  abstract = {{G}enome editing, which is an unprecedented technological breakthrough, has provided a valuable means of creating targeted mutations in plant genomes. {I}n this study, we developed a genomic web tool to identify all g{RNA} target sequences in the coffee genome, along with potential off-targets. {I}n all, 8,145,748 {CRISPR} guides were identified in the draft genome of {C}offea canephora corresponding to 5,338,568 different sequences and, of these, 4,655,458 were single, and 514,591 were covering exons. {T}he proof of concept was established by targeting the phytoene desaturase gene ({C}c{PDS}) using the {A}grobacterium tumefaciens transformation technique and somatic embryogenesis as the plant regeneration method. {A}n analysis of the {RNA}-guided genome-editing events showed that 22.8% of the regenerated plants were heterozygous mutants and 7.6% were homozygous mutants. {M}utation efficiency at the target site was estimated to be 30.4%. {W}e demonstrated that genome editing by the {CRISPR}/{C}as9 method is an efficient and reliable way of knocking out genes of agronomic interest in the coffee tree, opening up the way for coffee molecular breeding. {O}ur results also showed that the use of somatic embryogenesis, as the method for regenerating genome-edited plants, could restrict the choice of targeted genes to those that are not essential to the embryo development and germination steps.},
  keywords = {{CRISPR}/{C}as9 ; {C}offea canephora ; {P}hytoene desaturase ; {S}omatic embryogenesis ; {T}argeted mutagenesis},
  booktitle = {},
  journal = {{P}lant {C}ell {T}issue and {O}rgan {C}ulture},
  volume = {134},
  numero = {3},
  pages = {383--394},
  ISSN = {0167-6857},
  year = {2018},
  DOI = {10.1007/s11240-018-1429-2},
  URL = {https://www.documentation.ird.fr/hor/fdi:010073769},
}