@article{fdi:010073739,
  title = {{D}icer-like and {RNA}-dependent {RNA} polymerase gene family identification and annotation in the cultivated {S}olanum tuberosum and its wild relative {S}. commersonii},
  author = {{E}sposito, {S}. and {A}versano, {R}. and {D}'{A}melia, {V}. and {V}illano, {C}. and {A}lioto, {D}. and {M}irouze, {M}arie and {C}arputo, {D}.},
  editor = {},
  language = {{ENG}},
  abstract = {{W}e provide advances in {DCL} and {RDR} gene diversity in {S}olanaceae. {W}e also shed light on {DCL} and {RDR} gene expression in response to cold stress. {DICER}-like ({DCL}) and {RNA}-dependent {RNA} polymerase ({RDR}) genes form the core components to trigger small non-coding {RNA} (nc{RNA}) production. {I}n spite of this, little is known about the two gene families in non-model plant species. {A}s their genome sequences are now available, the cultivated potato ({S}olanum tuberosum) and its cold-tolerant wild relative {S}olanum commersonii offer a valuable opportunity to advance our understanding of the above genes. {T}o determine the extent of diversification and evolution of {DCL}s and {RDR}s in these species, we performed a comparative analysis. {S}even {DCL}s were identified in the two species, whereas seven and six {RDR} genes were found in {S}. tuberosum and {S}. commersonii, respectively. {B}ased on phylogenetic analysis with {DCL}s and {RDR}s from several species, we provide evidence for an increase in their number in both potato species. {W}e also disclosed that tandem duplications played a major role in the evolution of these gene families in {S}olanaceae. {DCL} and {RDR} expression was investigated in different tissues and under cold and virus stresses, with divergent profiles of the tandem duplicated genes being found in different tissues. {DCL} paralogs showed a contrasting expression in {S}. tuberosum and {S}. commersonii following cold stress and virus infection. {B}y contrast, no change in {RDR} transcript activity was detected following both stresses. {O}verall, this study provides the first comparative genomic analysis of the core components of the {RNA}i machinery in {S}olanaceae and offers a scaffold for future functional analysis of these gene families.},
  keywords = {{C}old stress ; {C}omparative genomics ; {G}ene silencing ; {M}icro{RNA} biogenesis ; {P}otato virus {Y}},
  booktitle = {},
  journal = {{P}lanta},
  volume = {248},
  numero = {3},
  pages = {729--743},
  ISSN = {0032-0935},
  year = {2018},
  DOI = {10.1007/s00425-018-2937-3},
  URL = {https://www.documentation.ird.fr/hor/fdi:010073739},
}