@article{fdi:010073730,
  title = {{F}unctional and genome sequence-driven characterization of {TAL} effector gene repertoires reveals novel variants with altered specificities in closely related {M}alian {X}anthomonas oryzae pv. oryzae strains},
  author = {{D}oucoure, {H}. and {P}erez-{O}uintero, {A}. {L}. and {R}eshetnyak, {G}. and {T}ekete, {C}. and {A}uguy, {F}lorence and {T}homas, {E}. and {K}oebnik, {R}alf and {S}zurek, {B}oris and {K}oita, {O}. and {V}erdier, {V}al{\'e}rie and {C}unnac, {S}{\'e}bastien},
  editor = {},
  language = {{ENG}},
  abstract = {{R}ice bacterial leaf blight ({BLB}) is caused by {X}anthomonas oryzae pv. oryzae ({X}oo) which injects {T}ranscription {A}ctivator-{L}ike {E}ffectors ({TALE}s) into the host cell to modulate the expression of target disease susceptibility genes. {X}oo major-virulence {TALE}s universally target susceptibility genes of the {SWEET} sugar transporter family. {TALE}-unresponsive alleles of {O}s{SWEET} genes have been identified in the rice germplasm or created by genome editing and confer resistance to {BLB}. {I}n recent years, {BLB} has become one of the major biotic constraints to rice cultivation in {M}ali. {T}o inform the deployment of alternative sources of resistance in this country, rice lines carrying alleles of {O}s{SWEET}14 unresponsive to either {T}al{F} (formerly {T}al5) or {T}al{C}, two important {TALE}s previously identified in {W}est {A}frican {X}oo, were challenged with a panel of strains recently isolated in {M}ali and were found to remain susceptible to these isolates. {T}he characterization of {TALE} repertoires revealed that tal{F} and tal{C} specific molecular markers were simultaneously present in all surveyed {M}alian strains, suggesting that the corresponding {TALE}s are broadly deployed by {M}alian {X}oo to redundantly target the {O}s{SWEET}14 gene promoter. {C}onsistent with this, the capacity of most {M}alian {X}oo to induce {O}s{SWEET}14 was unaffected by either tal{C}- or tal{F}-unresponsive alleles of this gene. {L}ong-read sequencing and assembly of eight {M}alian {X}oo genomes confirmed the widespread occurrence of active {T}al{F} and {T}al{C} variants and provided a detailed insight into the diversity of {TALE} repertoires. {A}ll sequenced strains shared nine evolutionary related tal effector genes. {N}otably, a new {T}al{F} variant that is unable to induce {O}s{SWEET}14 was identified. {F}urthermore, two distinct {T}al{B} variants were shown to have lost the ability to simultaneously induce two susceptibility genes as previously reported for the founding members of this group from strains {MAI}1 and {BAI}3. {Y}et, both new {T}al{B} variants retained the ability to induce one or the other of the two susceptibility genes. {T}hese results reveal molecular and functional differences in tal repertoires and will be important for the sustainable deployment of broad-spectrum and durable resistance to {BLB} in {W}est {A}frica.},
  keywords = {rice ; {X}anthomonas oryzae ; bacterial leaf blight ; {TAL} effector ; {M}ali ; disease resistance ; {MALI}},
  booktitle = {},
  journal = {{F}rontiers in {M}icrobiology},
  volume = {9},
  numero = {},
  pages = {1657 [17 ]},
  ISSN = {1664-302{X}},
  year = {2018},
  DOI = {10.3389/fmicb.2018.01657},
  URL = {https://www.documentation.ird.fr/hor/fdi:010073730},
}