Lembo-Fazio L., Billod J. M., Di Lorenzo F., Paciello I., Pallach M., Vaz-Francisco S., Holgado A., Beyaert R., Fresno M., Shimoyama A., Lanzetta R., Fukase K., Gully Djamel, Giraud Eric, Martin-Santamaria S., Bernardini M. L., Silipo A. (2018). Bradyrhizobium Lipid A : immunological properties and molecular basis of its binding to the myeloid differentiation protein-2/toll-like receptor 4 complex. Frontiers in Immunology, 9, p. art. 1888 [14 p.]. ISSN 1664-3224.
Titre du document
Bradyrhizobium Lipid A : immunological properties and molecular basis of its binding to the myeloid differentiation protein-2/toll-like receptor 4 complex
Année de publication
2018
Auteurs
Lembo-Fazio L., Billod J. M., Di Lorenzo F., Paciello I., Pallach M., Vaz-Francisco S., Holgado A., Beyaert R., Fresno M., Shimoyama A., Lanzetta R., Fukase K., Gully Djamel, Giraud Eric, Martin-Santamaria S., Bernardini M. L., Silipo A.
Source
Frontiers in Immunology, 2018,
9, p. art. 1888 [14 p.] ISSN 1664-3224
Lipopolysaccharides (LPS) are potent activator of the innate immune response through the binding to the myeloid differentiation protein-2 (MD-2)/toll-like receptor 4 (TLR4) receptor complexes. Although a variety of LPSs have been characterized so far, a detailed molecular description of the structure-activity relationship of the lipid A part has yet to be clarified. Photosynthetic Bradyrhizobium strains, symbiont of Aeschynomene legumes, express distinctive LPSs bearing very long-chain fatty acids with a hopanoid moiety covalently linked to the lipid A region. Here, we investigated the immunological properties of LPSs isolated from Bradyrhizobium strains on both murine and human immune systems. We found that they exhibit a weak agonistic activity and, more interestingly, a potent inhibitory effect on MD-2/TLR4 activation exerted by toxic enterobacterial LPSs. By applying computational modeling techniques, we also furnished a plausible explanation for the Bradyrhizobium LPS inhibitory activity at atomic level, revealing that its uncommon lipid A chemical features could impair the proper formation of the receptorial complex, and/or has a destabilizing effect on the pre-assembled complex itself.
Plan de classement
Sciences fondamentales / Techniques d'analyse et de recherche [020]
;
Santé : généralités [050]
;
Biotechnologies [084]
Localisation
Fonds IRD [F B010073729]
Identifiant IRD
fdi:010073729
