Horizon / Plein textes La base de ressources documentaires de l'IRD

IRD

Publications des scientifiques de l'IRD

Tran T. T., Perez-Quintero A. L., Wonni I., Carpenter S. C. D., Yu Y. H., Wang L., Leach J. E., Verdier Valérie, Cunnac Sébastien, Bogdanove A. J., Koebnik Ralf, Hutin M., Szurek Boris. (2018). Functional analysis of African Xanthomonas oryzae pv. oryzae TALomes reveals a new susceptibility gene in bacterial leaf blight of rice. PLoS Pathogens, 14 (6), e1007092 [25 p.]. ISSN 1553-7366

Fichier PDF disponible http://horizon.documentation.ird.fr/exl-doc/pleins_textes/divers18-09/010073703.pdf

Lien direct chez l'éditeur doi:10.1371/journal.ppat.1007092

Titre
Functional analysis of African Xanthomonas oryzae pv. oryzae TALomes reveals a new susceptibility gene in bacterial leaf blight of rice
Année de publication2018
Type de documentArticle référencé dans le Web of Science WOS:000437410000016
AuteursTran T. T., Perez-Quintero A. L., Wonni I., Carpenter S. C. D., Yu Y. H., Wang L., Leach J. E., Verdier Valérie, Cunnac Sébastien, Bogdanove A. J., Koebnik Ralf, Hutin M., Szurek Boris.
SourcePLoS Pathogens, 2018, 14 (6), p. e1007092 [25 p.]. p. e1007092 [25 p.] ISSN 1553-7366
RésuméMost Xanthomonas species translocate Transcription Activator-Like (TAL) effectors into plant cells where they function like plant transcription factors via a programmable DNA-binding domain. Characterized strains of rice pathogenic X. oryzae pv. oryzae harbor 9-16 different tal effector genes, but the function of only a few of them has been decoded. Using sequencing of entire genomes, we first performed comparative analyses of the complete repertoires of TAL effectors, herein referred to as TALomes, in three Xoo strains forming an African genetic lineage different from Asian Xoo. A phylogenetic analysis of the three TALomes combined with in silico predictions of TAL effector targets showed that African Xoo TALomes are highly conserved, genetically distant from Asian ones, and closely related to TAL effectors from the bacterial leaf streak pathogen Xanthomonas oryzae pv. oryzicola (Xoc). Nine clusters of TAL effectors could be identified among the three TALomes, including three showing higher levels of variation in their repeat variable diresidues (RVDs). Detailed analyses of these groups revealed recombination events as a possible source of variation among TAL effector genes. Next, to address contribution to virulence, nine TAL effector genes from the Malian Xoo strain MAI1 and four allelic variants from the Burkinabe Xoo strain BAI3, thus representing most of the TAL effector diversity in African Xoo strains, were expressed in the TAL effector-deficient X. oryzae strain X11-5A for gain-of-function assays. Inoculation of the susceptible rice variety Azucena lead to the discovery of three TAL effectors promoting virulence, including two TAL effectors previously reported to target the susceptibility (S) gene OsSWEET14 and a novel major virulence contributor, TalB. RNA profiling experiments in rice and in silico prediction of EBEs were carried out to identify candidate targets of TalB, revealing OsTFX1, a bZIP transcription factor previously identified as a bacterial blight S gene, and OsERF123, which encodes a subgroup IXc AP2/ERF transcription factor. Use of designer TAL effectors demonstrated that induction of either gene resulted in greater susceptibility to strain X11-5A. The induction of OsERF123 by BAI3 Delta 1, a talB knockout derivative of BAI3, carrying these designer TAL effectors increased virulence of BAI3 Delta 1, validating OsERF#123 as a new, bacterial blight S gene.
Plan de classementSciences du monde végétal [076] ; Biotechnologies [084]
LocalisationFonds IRD [F B010073703]
Identifiant IRDfdi:010073703
Lien permanenthttp://www.documentation.ird.fr/hor/fdi:010073703

Export des données

Disponibilité des documents

Télechargment fichier PDF téléchargeable

Lien sur le Web lien chez l'éditeur

Accès réservé en accès réservé

HAL en libre accès sur HAL


Accès aux documents originaux :

Le FDI est labellisé CollEx

Accès direct

Bureau du chercheur

Site de la documentation

Espace intranet IST (accès réservé)

Suivi des publications IRD (accès réservé)

Mentions légales

Services Horizon

Poser une question

Consulter l'aide en ligne

Déposer une publication (accès réservé)

S'abonner au flux RSS

Voir les tableaux chronologiques et thématiques

Centres de documentation

Bondy

Montpellier (centre IRD)

Montpellier (MSE)

Nouméa

Papeete

Niamey

Ouagadougou

Tunis

La Paz

Quito