@article{fdi:010073679,
  title = {{A} new phenotyping pipeline reveals three types of lateral roots and a random branching pattern in two cereals},
  author = {{P}assot, {S}. and {M}oreno-{O}rtega, {B}. and {M}oukouanga, {D}aniel and {B}alsera, {C}. and {G}uyomarc'h, {S}. and {L}ucas, {M}ika{\¨e}l and {L}obet, {G}. and {L}aplaze, {L}aurent and {M}uller, {B}. and {G}uedon, {Y}.},
  editor = {},
  language = {{ENG}},
  abstract = {{R}ecent progress in root phenotyping has focused mainly on increasing throughput for genetic studies, while identifying root developmental patterns has been comparatively underexplored. {W}e introduce a new phenotyping pipeline for producing high-quality spatiotemporal root system development data and identifying developmental patterns within these data. {T}he {S}mart{R}oot image-analysis system and temporal and spatial statistical models were applied to two cereals, pearl millet ({P}ennisetum glaucum) and maize ({Z}ea mays). {S}emi-{M}arkov switching linear models were used to cluster lateral roots based on their growth rate profiles. {T}hese models revealed three types of lateral roots with similar characteristics in both species. {T}he first type corresponds to fast and accelerating roots, the second to rapidly arrested roots, and the third to an intermediate type where roots cease elongation after a few days. {T}hese types of lateral roots were retrieved in different proportions in a maize mutant affected in auxin signaling, while the first most vigorous type was absent in maize plants exposed to severe shading. {M}oreover, the classification of growth rate profiles was mirrored by a ranking of anatomical traits in pearl millet. {P}otential dependencies in the succession of lateral root types along the primary root were then analyzed using variable-order {M}arkov chains. {T}he lateral root type was not influenced by the shootward neighbor root type or by the distance from this root. {T}his random branching pattern of primary roots was remarkably conserved, despite the high variability of root systems in both species. {O}ur phenotyping pipeline opens the door to exploring the genetic variability of lateral root developmental patterns.},
  keywords = {},
  booktitle = {},
  journal = {{P}lant {P}hysiology},
  volume = {177},
  numero = {3},
  pages = {896--910},
  ISSN = {0032-0889},
  year = {2018},
  DOI = {10.1104/pp.17.01648},
  URL = {https://www.documentation.ird.fr/hor/fdi:010073679},
}