%0 Journal Article
%9 ACL : Articles dans des revues avec comité de lecture répertoriées par l'AERES
%A Selles, Benjamin
%A Michaud, Caroline
%A Xiong, T. C.
%A Leblanc, Olivier
%A Ingouff, M.
%T Arabidopsis pollen tube germination and growth depend on the mitochondrial calcium uniporter complex
%D 2018
%L fdi:010073675
%G ENG
%J New Phytologist
%@ 0028-646X
%K Arabidopsis ; calcium ; mitochondrial calcium uniporter (MCU) ; plant reproduction ; pollen tube ; tip-growth
%M ISI:000434153200012
%N 1
%P 58-65
%R 10.1111/nph.15189
%U https://www.documentation.ird.fr/hor/fdi:010073675
%> https://www.documentation.ird.fr/intranet/publi/2018/08/010073675.pdf
%V 219
%W Horizon (IRD)
%X The mitochondrial calcium uniporter complex (MCUc) was recently characterized in details in metazoans and consists of pore-forming units (MCUs) and regulatory factors that channel calcium (Ca2+) ion into the mitochondria. MCUs participate in many stress and developmentally related processes involving Ca2+. Although multiple homologues of MCUs and one regulatory subunit are usually present in plants, the first functional characterization and contribution to Ca2+ related processes of these proteins have been reported recently. Here, we focused on two predicted Arabidopsis MCUs and studied their role in the germination and the growth of pollen tube, a tip-growing cell type highly dependent on Ca2+ homeostasis. Heterologous expression of MCU1 or MCU2 in yeast is sufficient to generate a mitochondrial Ca2+ influx. MCU1 and MCU2 fluorescent reporters are co-expressed in the vegetative cell mitochondria of the pollen grain but are undetectable in the embryo sac. We demonstrate that MCU1 and MCU2 can form a heterotypic complex. Phenotypic analyses revealed an impaired pollen tube germination and growth in vitro only for the mcu2 mutants suggesting a predominant role of MCU2. Our results show that mitochondrial Ca2+ controlled by MCUs is an additional player in Arabidopsis pollen tube germination and growth.
%$ 076 ; 020