Selles Benjamin, Michaud Caroline, Xiong T. C., Leblanc Olivier, Ingouff M. (2018). Arabidopsis pollen tube germination and growth depend on the mitochondrial calcium uniporter complex. New Phytologist, 219 (1), p. 58-65. ISSN 0028-646X.
Titre du document
Arabidopsis pollen tube germination and growth depend on the mitochondrial calcium uniporter complex
Selles Benjamin, Michaud Caroline, Xiong T. C., Leblanc Olivier, Ingouff M.
Source
New Phytologist, 2018,
219 (1), p. 58-65 ISSN 0028-646X
The mitochondrial calcium uniporter complex (MCUc) was recently characterized in details in metazoans and consists of pore-forming units (MCUs) and regulatory factors that channel calcium (Ca2+) ion into the mitochondria. MCUs participate in many stress and developmentally related processes involving Ca2+. Although multiple homologues of MCUs and one regulatory subunit are usually present in plants, the first functional characterization and contribution to Ca2+ related processes of these proteins have been reported recently. Here, we focused on two predicted Arabidopsis MCUs and studied their role in the germination and the growth of pollen tube, a tip-growing cell type highly dependent on Ca2+ homeostasis. Heterologous expression of MCU1 or MCU2 in yeast is sufficient to generate a mitochondrial Ca2+ influx. MCU1 and MCU2 fluorescent reporters are co-expressed in the vegetative cell mitochondria of the pollen grain but are undetectable in the embryo sac. We demonstrate that MCU1 and MCU2 can form a heterotypic complex. Phenotypic analyses revealed an impaired pollen tube germination and growth in vitro only for the mcu2 mutants suggesting a predominant role of MCU2. Our results show that mitochondrial Ca2+ controlled by MCUs is an additional player in Arabidopsis pollen tube germination and growth.
Plan de classement
Sciences fondamentales / Techniques d'analyse et de recherche [020]
;
Sciences du monde végétal [076]
