%0 Journal Article
%9 ACL : Articles dans des revues avec comité de lecture répertoriées par l'AERES
%A Lopez-Ramirez, N.
%A Volke-Sepulveda, T.
%A Perraud Gaime, Isabelle
%A Saucedo-Castaneda, G.
%A Favela-Torres, E.
%T Effect of stirring on growth and cellulolytic enzymes production by Trichoderma harzianum in a novel bench-scale solid-state fermentation bioreactor
%D 2018
%L fdi:010073648
%G ENG
%J Bioresource Technology
%@ 0960-8524
%K Solid-state fermentation ; Stirred bioreactor ; Respirometry ; Trichoderma harzianum ; Cellulases ; Xylanases
%M ISI:000439467300035
%P 291-298
%R 10.1016/j.biortech.2018.06.015
%U https://www.documentation.ird.fr/hor/fdi:010073648
%> https://www.documentation.ird.fr/intranet/publi/2018/08/010073648.pdf
%V 265
%W Horizon (IRD)
%X A novel bench-scale stirred bioreactor for solid-state fermentation was used to determine the effect of the stirring rate on growth and enzymes production by Trichoderma harzianum PBLA. Lab-scale static tubular bioreactors were first used to assess the effect of bioreactor diameter on heat accumulation, growth, and production of cellulases and xylanases. The increased diameters (1.8-4.2 cm) led to increases in temperature up to 36 degrees C (at a rate of 1.08 degrees C/cm), which negatively affected the growth and enzyme production. Afterward, in the bench-scale bioreactor operated at rates up to 3.0 rpm, maximum xylanases production (107 +/- 0.3 U/g dm) was attained at rates of 0.5 and 1.0 rpm, reaching a maximum of 34 +/- 0.3 degrees C. Cellulases production was reduced (up to 79%) due to stirring. Therefore, the production of xylanases by T. harzianum can be performed in this cross-flow stirred SSF bioreactor at rates up to 1.0 rpm, avoiding heat accumulation and damage on metabolic activity.
%$ 084