@article{fdi:010073619,
  title = {{B}lood cell disruption to significantly improve the {B}orrelia {PCR} detection sensitivity in borreliosis in humans},
  author = {{L}acout, {A}. and {M}one, {Y}. and {F}ranck, {M}. and {M}arcy, {P}. {Y}. and {M}as, {M}. and {V}eas, {F}rancisco and {P}erronne, {C}.},
  editor = {},
  language = {{ENG}},
  abstract = {{L}yme disease is the most frequently reported zoonotic tick-borne disease worldwide, and the number of infected humans is increasing. {L}yme disease (or {L}yme borreliosis) is an affection caused by the spirochete {B}orrelia burgdorferi, sensu lato. {L}yme disease is also reported as a variety of misleading clinical symptomatologies. {I}nfected patient's blood serology is the most currently test used for its diagnosis. {H}owever, serology has a low sensitivity, which ranges from 34% to 70%. {T}hus, there are numerous subsequent false-negative diagnoses despite an active clinical infection profile. {T}herefore, alternative and more sensitive techniques are required to detect the antigens or nucleic acids of {B}orrelia. {A}ctually, the most appropriate methodological approach seems to be the polymerase chain reaction ({PCR}). {H}owever, {PCR} will detect the only "visible" part available of the targeted {DNA} presence in the blood of the infected patients. {C}onsequently {PCR} alone will not be conclusive enough to reach the final diagnosis. {C}onsidering the ability of {B}orrelia to invade host cells, we hypothesize that a selective lysis of all blood cells should improve the diagnostic sensitivity of the detection of {B}orrelia by {PCR} in whole blood, and subsequently reduce the false-negative diagnostic rate, thus improving the patient's diagnosis and therapeutic management.},
  keywords = {{L}yme ; {B}orrelia ; {B}lood cell lysis ; {PCR}},
  booktitle = {},
  journal = {{M}edical {H}ypotheses},
  volume = {116},
  numero = {},
  pages = {1--3},
  ISSN = {0306-9877},
  year = {2018},
  DOI = {10.1016/j.mehy.2018.04.012},
  URL = {https://www.documentation.ird.fr/hor/fdi:010073619},
}