Waltmann A., Patel J. C., Thwai K. L., Hathaway N. J., Parobek C. M., Massougbodji A., Fievet Nadine, Bailey J. A., Deloron Philippe, Juliano J. J., Tuikue Ndam Nicaise, Meshnick S. R. (2018). Matched placental and circulating Plasmodium falciparum parasites are genetically homologous at the var2csa ID1-DBL2X locus by deep sequencing. American Journal of Tropical Medicine and Hygiene, 98 (1), p. 77-82. ISSN 0002-9637.
Titre du document
Matched placental and circulating Plasmodium falciparum parasites are genetically homologous at the var2csa ID1-DBL2X locus by deep sequencing
Année de publication
2018
Auteurs
Waltmann A., Patel J. C., Thwai K. L., Hathaway N. J., Parobek C. M., Massougbodji A., Fievet Nadine, Bailey J. A., Deloron Philippe, Juliano J. J., Tuikue Ndam Nicaise, Meshnick S. R.
Source
American Journal of Tropical Medicine and Hygiene, 2018,
98 (1), p. 77-82 ISSN 0002-9637
In pregnancy-associated malaria, infected erythrocytes accumulate in the placenta. It is unclear if in polyclonal infections this results in distinct peripheral and placental parasite populations. We used long amplicon deep sequencing of Plasmodium falciparum var2csa ID1-DBL2X from 15 matched peripheral and placental samples collected at delivery from a high transmission area to determine genetic homology. Despite substantial sequence variation and detecting 23 haplotypes, the matched pairs mostly contained the same genetic variants, with 11 pairs sharing 100% of their variants, whereas others showed some heterogeneity. Thus, at delivery, peripheral and placental parasites appear to intermix and placental genotypes can be inferred through peripheral sampling.
Plan de classement
Sciences fondamentales / Techniques d'analyse et de recherche [020]
;
Entomologie médicale / Parasitologie / Virologie [052]
Description Géographique
BENIN
Localisation
Fonds IRD [F B010072817]
Identifiant IRD
fdi:010072817
