@article{fdi:010072795,
  title = {{D}ynamic succession of chickpea rhizobia over years and sampling sites in {X}injiang, {C}hina},
  author = {{Z}hang, {J}. {J}. and {G}uo, {C}. and {C}hen, {W}. {F}. and {S}hang, {Y}. {M}. and {L}ajudie, {P}hilippe de and {Y}ang, {X}. and {M}ao, {P}. {H}. and {Z}heng, {J}. {Q}. and {W}ang, {E}. {T}.},
  editor = {},
  language = {{ENG}},
  abstract = {{A}ims {T}o investigate the succession of chickpea rhizobia over years and sampling sites. {M}ethods {R}hizobia were sampled four times from nodules of chickpea grown in four sites of {M}ulei {C}ounty and {Q}itai {C}ounty in {X}injiang, {C}hina during 2009-2016. {T}he rec{A} genes of rhizobia representing different populations were phylogenetically compared for their genotypic differentiation. {S}oil properties were examined to explore their hypothetical incidence on the rhizobial succession. {R}esults {T}he 628 isolates were all identified as {M}esorhizobium muleiense, belonging to 28 genotypes distinguished by rec{A} phylogeny. {F}or each site, 15, 17, 10 and 7 genotypes were detected during the whole sampling period; while 4, 15, 12 and 17 genotypes were evidenced in each of the 4 sampling years (2009, 2013, 2015 and 2016) from all the four sites. {F}our major genotypes ({T}12 through {T}15) were found throughout the four years in the four sites at various frequencies. {W}hile the other genotypes were site and/or year specific. {B}oth increased genetic diversity as revealed by {S}hannon-{W}eaver index and shifts in the population composition as revealed by genotyping were detected in the chickpea rhizobial populations during the study period. {N}itrogen content, available phosphorus and potassium, and p{H} were the main factors correlated to the genotype distribution of chickpea rhizobia in the soil over years. {C}onclusions {T}he genetic diversity of {M}. muleiense increased and the population composition shifted over years and at different sampling sites. {T}he rec{A} gene is an adequate molecular marker to estimate the intraspecific variation and succession of {M}. muleiense genotypes. {T}he rhizobial genotype succession/evolution in the fields might be driven by the fluctuations of soil characteristics like nitrogen content, available phosphorus and potassium, and p{H}.},
  keywords = {{C}hickpea ; {M}esorhizobium muleiense ; {R}hizobium ; {G}enotypes ; {S}uccession ; {CHINE}},
  booktitle = {},
  journal = {{P}lant and {S}oil},
  volume = {425},
  numero = {1-2},
  pages = {241--251},
  ISSN = {0032-079{X}},
  year = {2018},
  DOI = {10.1007/s11104-018-3586-9},
  URL = {https://www.documentation.ird.fr/hor/fdi:010072795},
}