@article{fdi:010072758,
  title = {{S}ub-cellular markers highlight intracellular dynamics of membrane proteins in response to abiotic treatments in rice},
  author = {{C}hu, {T}. {T}. {H}. and {H}oang, {T}. {G}. and {T}rinh, {D}. {C}. and {B}ureau, {C}. and {M}eynard, {D}. and {V}ernet, {A}. and {I}ngouff, {M}. and {D}o, {N}. {V}. and {P}erin, {C}. and {G}uiderdoni, {E}. and {G}antet, {P}ascal and {M}aurel, {C}. and {L}uu, {D}. {T}.},
  editor = {},
  language = {{ENG}},
  abstract = {{B}ackground: {C}ell biology approach using membrane protein markers tagged with fluorescent proteins highlights the dynamic behaviour of plant cell membranes, not only in the standard but also in changing environmental conditions. {I}n the past, this strategy has been extensively developed in plant models such as {A}rabidopsis. {R}esults: {H}ere, we generated a set of transgenic lines expressing membrane protein markers to extend this approach to rice, one of the most cultivated crop in the world and an emerging plant model. {L}ines expressing individually eight membrane protein markers including five aquaporins ({O}s{PIP}1; 1, {O}s{PIP}2; 4, {O}s{PIP}2; 5, {O}s{TIP}1; 1, {O}s{TIP}2; 2) and three endosomal trafficking proteins ({O}s{R}ab5a, {O}s{GAP}1, {O}s{SCAMP}1) were obtained. {I}mportantly, we challenged in roots the aquaporin-expressing transgenic lines upon salt and osmotic stress and uncovered a highly dynamic behaviour of cell membrane. {C}onclusion: {W}e have uncovered the relocalization and dynamics of plasma membrane aquaporins upon salt and osmotic stresses in rice. {I}mportantly, our data support a model where relocalization of {O}s{PIP}s is concomitant with their high cycling dynamics.},
  keywords = {{O}ryza sativa ; {S}ubcellular markers ; {I}ntracellular dynamics ; {A}biotic stress},
  booktitle = {},
  journal = {{R}ice},
  volume = {11},
  numero = {},
  pages = {art. 23 [8 p.]},
  ISSN = {1939-8425},
  year = {2018},
  DOI = {10.1186/s12284-018-0209-2},
  URL = {https://www.documentation.ird.fr/hor/fdi:010072758},
}