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Osdaghi E., Ansari M., Taghavi S. M., Zarei S., Koebnik Ralf, Lamichhane J. R. (2018). Pathogenicity and phylogenetic analysis of Clavibacter michiganensis strains associated with tomato plants in Iran. Plant Pathology, 67 (4), 957-970. ISSN 0032-0862

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Lien direct chez l'éditeur doi:10.1111/ppa.12801

Titre
Pathogenicity and phylogenetic analysis of Clavibacter michiganensis strains associated with tomato plants in Iran
Année de publication2018
Type de documentArticle référencé dans le Web of Science WOS:000429578000020
AuteursOsdaghi E., Ansari M., Taghavi S. M., Zarei S., Koebnik Ralf, Lamichhane J. R.
SourcePlant Pathology, 2018, 67 (4), p. 957-970. ISSN 0032-0862
RésuméDuring 2013-2016, 277 tomato fields were surveyed across Iran to monitor the status of bacterial canker of tomato, caused by Clavibacter michiganensis subsp. michiganensis. Altogether, 450 plant samples were collected, both with and without symptoms, from which 35 bacterial strains were recovered. These were positive for the PCR test performed using the Clavibacter-specific primer pair CMR16F1/CMR16R1. Based on the phylogeny of the gyrB gene sequences, 31, three and one of the 35 strains were identified as C. michiganensis, Microbacterium sp. and Agrococcus sp., respectively. The 31 strains of C. michiganensis were further identified as C. michiganensis subsp. michiganensis (23 strains), C. michiganensis subsp. tessellarius (six strains) and Clavibacter spp. (two strains). This was subsequently confirmed by multilocus sequence analysis (MLSA) of five housekeeping genes (atpD, gyrB, ppk, recA and rpoB). In pathogenicity tests, all 23 strains induced wilting symptoms on tomato plants in greenhouse conditions, while no symptoms were observed on eggplant, bell pepper and chili pepper plants. All evaluated pathogenicity determinant genes (celA, pat-1, tomA, ppaA, chpC and chpG) were detected in 18 out of 31 C. michiganensis strains, using eight specific primer pairs. Estimation of the number of nucleotide differences, sequence similarity matrix and MLSA clustered two peach-coloured strains (Tom495 and Tom532) separately from all nine previously described subspecies, thereby suggesting these two strains are a new subspecies of C. michiganensis. However, a detailed taxonomic study using multiphased molecular approaches is needed to delineate a formal taxonomic name for these atypical strains.
Plan de classementSciences du monde végétal [076]
Descr. géo.IRAN
LocalisationFonds IRD [F B010072755]
Identifiant IRDfdi:010072755
Lien permanenthttp://www.documentation.ird.fr/hor/fdi:010072755

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