@article{fdi:010072722, title = {{D}iverse laboratory colonies of {A}edes aegypti harbor the same adult midgut bacterial microbiome}, author = {{D}ickson, {L}. {B}. and {G}hozlane, {A}. and {V}olant, {S}. and {B}ouchier, {C}. and {M}a, {L}. and {V}ega-{R}ua, {A}. and {D}usfour, {I}. and {J}iolle, {D}. and {P}aupy, {C}hristophe and {M}ayanja, {M}. {N}. and {K}ohl, {A}. and {L}utwama, {J}. {J}. and {D}uong, {V}. and {L}ambrechts, {L}.}, editor = {}, language = {{ENG}}, abstract = {{B}ackground: {H}ost-associated microbes, collectively known as the microbiota, play an important role in the biology of multicellular organisms. {I}n mosquito vectors of human pathogens, the gut bacterial microbiota influences vectorial capacity and has become the subject of intense study. {I}n laboratory studies of vector biology, genetic effects are often inferred from differences between geographically and genetically diverse colonies of mosquitoes that are reared in the same insectary. {I}t is unclear, however, to what extent genetic effects can be confounded by uncontrolled differences in the microbiota composition among mosquito colonies. {T}o address this question, we used 16{S} metagenomics to compare the midgut bacterial microbiome of six laboratory colonies of {A}edes aegypti recently derived from wild populations representing the geographical range and genetic diversity of the species. {R}esults: {W}e found that the diversity, abundance, and community structure of the midgut bacterial microbiome was remarkably similar among the six different colonies of {A}e. aegypti, regardless of their geographical origin. {W}e also confirmed the relatively low complexity of bacterial communities inhabiting the mosquito midgut. {C}onclusions: {O}ur finding that geographically diverse colonies of {A}e. aegypti reared in the same insectary harbor a similar gut bacterial microbiome supports the conclusion that the gut microbiota of adult mosquitoes is environmentally determined regardless of the host genotype. {T}hus, uncontrolled differences in microbiota composition are unlikely to represent a significant confounding factor in genetic studies of vector biology.}, keywords = {{M}osquito ; {M}icrobiota ; {V}ectorial capacity ; {M}etagenomics ; {AUSTRALIE} ; {CAMBODGE} ; {GUYANE} {FRANCAISE} ; {GABON} ; {GUADELOUPE} ; {OUGANDA} ; {ZONE} {TROPICALE}}, booktitle = {}, journal = {{P}arasites and {V}ectors}, volume = {11}, numero = {}, pages = {art. 207 [8 p.]}, ISSN = {1756-3305}, year = {2018}, DOI = {10.1186/s13071-018-2780-1}, URL = {https://www.documentation.ird.fr/hor/fdi:010072722}, }