@article{fdi:010072718,
  title = {{I}ntegrative analysis of the late maturation programme and desiccation tolerance mechanisms in intermediate coffee seeds},
  author = {{D}ussert, {S}t{\'e}phane and {S}erret, {J}ulien and {B}astos-{S}iqueira, {A}. and {M}orcillo, {F}. and {D}echamp, {E}. and {R}ofidal, {V}. and {L}ashermes, {P}hilippe and {E}tienne, {H}. and {J}o{\¨e}t, {T}hierry},
  editor = {},
  language = {{ENG}},
  abstract = {{T}he 'intermediate seed' category was defined in the early 1990s using coffee ({C}offea arabica) as a model. {I}n contrast to orthodox seeds, intermediate seeds cannot survive complete drying, which is a major constraint for seed storage and has implications for both biodiversity conservation and agricultural purposes. {H}owever, intermediate seeds are considerably more tolerant to drying than recalcitrant seeds, which are highly sensitive to desiccation. {T}o gain insight into the mechanisms governing such differences, changes in desiccation tolerance ({DT}), hormone contents, and the transcriptome were analysed in developing coffee seeds. {A}cquisition of {DT} coincided with a dramatic transcriptional switch characterised by the repression of primary metabolism, photosynthesis, and respiration, and the up-regulation of genes coding for late-embryogenesis abundant ({LEA}) proteins, heat-shock proteins ({HSP}s), and antioxidant enzymes. {A}nalysis of the heat-stable proteome in mature coffee seeds confirmed the accumulation of {LEA} proteins identified at the transcript level. {T}ranscriptome analysis also suggested a major role for {ABA} and for the transcription factors {C}a{HSFA}9, {C}a{DREB}2{G}, {C}a{ANAC}029, {C}a{PLATZ}, and {C}a{DOG}-like in {DT} acquisition. {T}he ability of {C}a{HSFA}9 and {C}a{DREB}2{G} to trigger {HSP} gene transcription was validated by {A}grobacterium-mediated transformation of coffee somatic embryos.},
  keywords = {{A}grobacterium transformation ; {C}offea arabica ; desiccation tolerance ; heat-stable proteome ; intermediate seed ; late-embryogenesis abundant ; protein ; late maturation ; heat-shock factor ; heat-shock protein ; transcriptome},
  booktitle = {},
  journal = {{J}ournal of {E}xperimental {B}otany},
  volume = {69},
  numero = {7},
  pages = {1583--1597},
  ISSN = {0022-0957},
  year = {2018},
  DOI = {10.1093/jxb/erx492},
  URL = {https://www.documentation.ird.fr/hor/fdi:010072718},
}