@article{fdi:010072711,
  title = {{R}ecovery of oil palm ({E}laeis guineensis {J}acq.) somatic embryos cryostored for 20 years},
  author = {{B}eul{\'e}, {T}. and {I}lbert, {P}. and {A}deoti, {K}. and {D}urand-{G}asselin, {T}. and {D}umet, {D}ominique and {E}ngelmann, {F}lorent and {M}orcillo, {F}.},
  editor = {},
  language = {{ENG}},
  abstract = {{BACKGROUND}: {A} cryopreservation protocol has been established for oil palm somatic embryos ({SE}s), the efficiency of which must be evaluated, both in terms of regeneration and of long-term storage capacity, before its large-scale routine use. {OBJECTIVE}: {T}o test the survival and recovery of 29 clones of oil palm somatic embryos cryostored for 20 years. {MATERIALS} {AND} {METHODS}: {C}lumps of {SE}s were pregrown for 7 days on medium containing 0.75 {M} sucrose, dehydrated in air-tight containers containing silica gel to moisture contents between 19-35% fresh weight, and then immersed directly in liquid nitrogen and stored in cryotanks for 20 years. {RESULTS}: {S}urvival of {SE}s cryopreserved and rewarmed immediately displayed an average value of 19.1% for the 29 clones tested while survival of {SE}s rewarmed after 20 years of cryostorage was significantly higher, with an average of 33.2% for the 28 surviving clones. {O}ut of these 28 surviving clones, three were lost due to contamination or regrowth decline, six produced only shoots and the rest proliferated. {CONCLUSION}: {I}t is possible to cryostore oil palm {SE}s for extended periods and to regenerate proliferating cultures and plantlets from the cryopreserved material. {T}he cryopreservation protocol established can thus be efficiently used to store oil palm germplasm and to manage large-scale production in industrial laboratories.},
  keywords = {cryopreservation ; {E}laeis guineensis ; somatic embryos ; tissue-culture ; germplasm ; preservation},
  booktitle = {},
  journal = {{C}ryoletters},
  volume = {39},
  numero = {1},
  pages = {60--66},
  ISSN = {0143-2044},
  year = {2018},
  URL = {https://www.documentation.ird.fr/hor/fdi:010072711},
}