@article{fdi:010072669,
  title = {{I}mprovement of secondary metabolites from {P}hyllanthus odontadenius against malaria by mutagenesis},
  author = {{N}diku, {S}. {L}. and {N}akweti, {R}. {K}. and {S}inou, {V}. and {R}ouvier, {F}. and {F}undu, {T}. {M}. and {F}ranche, {C}laudine and {K}anianga, {R}. {C}.},
  editor = {},
  language = {{ENG}},
  abstract = {{A}ims: {M}ajority of deaths in children aged under 5 years are due to {P}lasmodium falciparum malaria. {M}alaria deaths in children decreased but malaria remains a major killer of children, taking the life of a child every 2 minutes. {T}his study aims to investigate the increasing of the in vitro antiplasmodial activities by mutagenesis techniques using gamma-rays ({C}s-137) or sodium azide ({N}a{N}3) as mutagens. {I}t will allow the importance of mutagenesis use as tools for improvement of secondary metabolites against malaria parasites using chemical or physical mutagens. {S}tudy {D}esign: {L}aboratory experiment tests : identification of plant material, immersion of seeds in {SA} (sodium azide) solutions or irradiation by {G}amma-rays ({C}s-137) of {P}. odontadenius seeds for improvement of secondary metabolites against malaria parasites, in vitro culture of seeds followed by the in situ culturing of plantlets for obtaining material of study, phytochemical screening of {P}hyllanthus odontadenius aerial parts to determine the change of compounds in comparison to controls, in vitro antiplasmodial tests for the determination of {SA} concentrations or those of gamma-rays doses which killing 50% of malaria parasite populations ({IC}50). {P}lace and {D}uration of {S}tudy: {D}epartment of {B}iotechnology and {M}olecular {B}iology, {D}epartment of {B}iochemistry: {G}eneral {A}tomic {E}nergy {C}ommission, {R}egional {N}uclear {S}tudies {C}enter of {K}inshasa, {P}.{O}.{B}ox. 868 {K}inshasa {XI}, {D}emocratic {R}epublic of the {C}ongo ({DRC}). {N}ational {I}nstitute of {B}iomedical {R}esearch ({NIBR}) at {K}inshasa/{G}ombe ({DRC}). {L}aboratory of {UMR}-{MD}3, {A}ix-{M}arseille {U}niversity, {F}aculty of {P}harmacy/{M}arseille, {F}rance. {T}he experiments were conducted from {J}unuary - {D}ecember 2010; {A}ugust and {D}ecember 2011 and during {M}ay and {S}eptember 2012 until {J}uly 2014. {M}ethodology: {A}erial parts of plants {M}1 and {M}2 from {G}amma-rays irradiation of {P}. odontadenius seeds or from immersion of {P}. odontadenius seeds in sodium azide solutions used as biological material for the in vitro antiplasmodial analysis. {T}he in vitro antiplasmodial activities assays on clinical isolates of {P}. falciparum and on chloroquine-resistante {P}. falciparum strain {K}1 was determined using microscopic method, the isotopic micro-test method and using {HRP}2-based {ELISA} assay. {R}esults: {G}amma-rays ({C}s-137) increased (multiplied) the in vitro antiplasmodial activities from 2.48 up to 7.6 in comparison to control. {T}hus, the in vitro antiplasmodial activities were improved or exceeded from 147.57% up to 660% than those of control plant. {SA} had increased (multiplied) the in vitro antiplasmodial activities from 1.24 up to 10.15 comparing to the control plants. {T}he antiplasmodial activities in vitro were exceeded compared to the control plants from 24.43% up to 915%. {C}onclusion: {T}reatment of {P}. odontadenius seeds by {G}amma-rays or by {SA} give plants high in vitro antiplasmodial activities. {V}alues of in vitro antiplasmodial activities varried from 1.24 (147.57%) to 10.15 (915%). 125, 150 and 225 {G}y of {G}amma-rays ({C}s-137) for physical mutagenesis and 10.15 and 17.5 m{M} of {SA} solutions for the chemical mutagenesis could be used for improving in vitro antiplasmodial activities against {P}. falciparum (clinical isolates of {P}. falciparum or chloroquine-resistant {P}. falciparum strain {K}1). {T}hus, plants extracts from treated seeds have justified the usefulness of mutagens in plant breeding particularly in the increasing production of secondary metabolite against malaria parasite.},
  keywords = {{M}utagenesis ; gamma rays ; sodium azide ; secondary metabolites ; {P}hyllanthus odontadenius ; malaria disease, antiplasmodial in vitro},
  booktitle = {},
  journal = {{B}ritish {J}ournal of {P}harmaceutical {R}esearch},
  volume = {21},
  numero = {2},
  pages = {art. 37978 [14 p.]},
  ISSN = {2231-2919},
  year = {2018},
  DOI = {10.9734/jpri/2018/37978},
  URL = {https://www.documentation.ird.fr/hor/fdi:010072669},
}