@article{fdi:010072416,
  title = {{C}iguatoxins activate the {C}alcineurin signalling pathway in yeasts : potential for development of an alternative detection tool ?},
  author = {{M}artin-{Y}ken, {H}. and {G}ironde, {C}. and {D}erick, {S}. and {D}arius, {H}. {T}. and {F}urger, {C}. and {L}aurent, {D}ominique and {C}hinain, {M}.},
  editor = {},
  language = {{ENG}},
  abstract = {{C}iguatoxins ({CTX}s) are lipid-soluble polyether compounds produced by dinoflagellates from the genus {G}ambierdiscus spp. typically found in tropical and subtropical zones. {T}his endemic area is however rapidly expanding due to environmental perturbations, and both toxic {G}ambierdiscus spp. and ciguatoxic fishes have been recently identified in the {N}orth {A}tlantic {O}cean ({M}adeira and {C}anary islands) and {M}editerranean {S}ea. {C}iguatoxins bind to {V}oltage {G}ated {S}odium {C}hannels on the membranes of sensory neurons, causing {C}iguatera {F}ish {P}oisoning ({CFP}) in humans, a disease characterized by a complex array of gastrointestinal, neurological, neuropsychological, and cardiovascular symptoms. {A}lthough {CFP} is the most frequently reported non bacterial food-borne poisoning worldwide, there is still no simple and quick way of detecting {CTX}s in contaminated samples. {I}n the prospect to engineer rapid and easy-to-use {CTX}s live cells-based tests, we have studied the effects of {CTX}s on the yeast {S}accharomyces cerevisiae, a unicellular model which displays a remarkable conservation of cellular signalling pathways with higher eukaryotes. {T}aking advantage of this high level of conservation, yeast strains have been genetically modified to encode specific transcriptional reporters responding to {CTX}s exposure. {T}hese yeast strains were further exposed to different concentrations of either purified {CTX} or micro-algal extracts containing {CTX}s. {O}ur data establish that {CTX}s are not cytotoxic to yeast cells even at concentrations as high as 1 mu {M}, and cause an increase in the level of free intracellular calcium in yeast cells. {C}oncomitantly, a dose dependent activation of the calcineurin signalling pathway is observed, as assessed by measuring the activity of specific transcriptional reporters in the engineered yeast strains. {T}hese findings offer promising prospects regarding the potential development of a yeast cells-based test that could supplement or, in some instances, replace current methods for the routine detection of {CTX}s in seafood products.},
  keywords = {{C}iguatoxins ; {Y}easts ; {B}ioassays ; {D}etection ; {C}alcineurin pathway},
  booktitle = {},
  journal = {{E}nvironmental {R}esearch},
  volume = {162},
  numero = {},
  pages = {144--151},
  ISSN = {0013-9351},
  year = {2018},
  DOI = {10.1016/j.envres.2017.12.022},
  URL = {https://www.documentation.ird.fr/hor/fdi:010072416},
}