%0 Journal Article
%9 ACL : Articles dans des revues avec comité de lecture répertoriées par l'AERES
%A Gannoun, H.
%A Ben Gaida, L.
%A Saidi, R.
%A Miladi, B.
%A Hamdi, M.
%A Bouallagui, H.
%A Liebgott, P. P.
%A Auria, Richard
%T A simple gas pressure manometer for measuring hydrogen production by hydrogenogenic cultures in serum bottles
%D 2018
%L fdi:010072361
%G ENG
%J Process Biochemistry
%@ 1359-5113
%K Hydrogen ; Total pressure ; Manometer ; Hydrogenogenic microorganisms
%M ISI:000425200200019
%P 157-163
%R 10.1016/j.procbio.2017.10.011
%U https://www.documentation.ird.fr/hor/fdi:010072361
%> https://www.documentation.ird.fr/intranet/publi/2018/03/010072361.pdf
%V 65
%W Horizon (IRD)
%X This study investigated the determination of hydrogen production by three H-2-producing microorganisms (Thermotoga maritima, Thermococcus kodakarensis KOD1 and Enterobacter cloacae) cultured in 116-mL serum bottles. A gas pressure manometer was used to measure total pressure in the serum-bottle headspace. It was demonstrated that total pressure is the sum of the saturation pressure of water, the pressure expansion of gases, and the partial pressures of H-2 (PgH2) and CO2 (PgCO2). A linear relationship was established between the partial pressure of H-2 measured by gas chromatography and the sum of the partial pressures of H-2 and CO2 measured by the manometer. When pH of culture medium was not controlled (pH decreased from 7 to 5), the PgH2/PgCO2 ratio was close to stoichiometric H-2/CO2 yield ratio of the most plausible metabolic pathways of each strain. PgH2/PgCO2 values were 1.7, 1.9 and 0.9 for T. maritima, T. kodakarensis and E. cloacae, respectively. In these experimental conditions, PgH2 can be deduced from total pressure measured by manometer.
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