Gannoun H., Ben Gaida L., Saidi R., Miladi B., Hamdi M., Bouallagui H., Liebgott P. P., Auria Richard. (2018). A simple gas pressure manometer for measuring hydrogen production by hydrogenogenic cultures in serum bottles. Process Biochemistry, 65, p. 157-163. ISSN 1359-5113.
Titre du document
A simple gas pressure manometer for measuring hydrogen production by hydrogenogenic cultures in serum bottles
Gannoun H., Ben Gaida L., Saidi R., Miladi B., Hamdi M., Bouallagui H., Liebgott P. P., Auria Richard
Source
Process Biochemistry, 2018,
65, p. 157-163 ISSN 1359-5113
This study investigated the determination of hydrogen production by three H-2-producing microorganisms (Thermotoga maritima, Thermococcus kodakarensis KOD1 and Enterobacter cloacae) cultured in 116-mL serum bottles. A gas pressure manometer was used to measure total pressure in the serum-bottle headspace. It was demonstrated that total pressure is the sum of the saturation pressure of water, the pressure expansion of gases, and the partial pressures of H-2 (PgH2) and CO2 (PgCO2). A linear relationship was established between the partial pressure of H-2 measured by gas chromatography and the sum of the partial pressures of H-2 and CO2 measured by the manometer. When pH of culture medium was not controlled (pH decreased from 7 to 5), the PgH2/PgCO2 ratio was close to stoichiometric H-2/CO2 yield ratio of the most plausible metabolic pathways of each strain. PgH2/PgCO2 values were 1.7, 1.9 and 0.9 for T. maritima, T. kodakarensis and E. cloacae, respectively. In these experimental conditions, PgH2 can be deduced from total pressure measured by manometer.
