@article{fdi:010072341,
  title = {{I}n vivo validation of anti-malarial activity of crude extracts of {T}erminalia macroptera, a {M}alian medicinal plant},
  author = {{H}aidara, {M}. and {H}addad, {M}ohamed and {D}enou, {A}. and {M}arti, {G}. and {B}ourgeade-{D}elmas, {S}andra and {S}anogo, {R}. and {B}ourdy, {G}enevi{\`e}ve and {A}ubouy, {A}gn{\`e}s},
  editor = {},
  language = {{ENG}},
  abstract = {{B}ackground: {P}lasmodium falciparum malaria is still one of the most deadly pathology worldwide. {E}fficient treatment is jeopardized by parasite resistance to artemisinin and its derivatives, and by poor access to treatment in endemic regions. {A}nti-malarial traditional remedies still offer new tracks for identifying promising antiplasmodial molecules, and a way to ensure that all people have access to care. {T}he present study aims to validate the traditional use of {T}erminalia macroptera, a {M}alian plant used in traditional medicine. {M}ethods: {T}erminalia macroptera was collected in {M}ali. {L}eaves ({TML}) and roots ethanolic extracts ({TMR}) were prepared and tested at 2000 mg/kg for in vivo acute toxicity in {A}lbino {S}wiss mice. {A}ntiplasmodial activity of the extracts was assessed against a chloroquine resistant strain {P}. falciparum ({F}c{B}1) in vitro. {I}n vivo, anti-malarial efficacy was assessed by a 4-day suppressive test at 100 mg/kg in two malaria murine models of uncomplicated malaria ({P}lasmodium chabaudi chabaudi infection) and cerebral malaria ({P}lasmodium berghei strain {ANKA} infection). {C}onstituents of {TMR} were characterized by ultra-high-performance liquid chromatography coupled to high resolution mass spectrometry. {T}op ranked compounds were putatively identified using plant databases and in silico fragmentation pattern. {R}esults: {L}ethal dose of {TML} and {TMR} were greater than 2000 mg/kg in {A}lbino {S}wiss mice. {A}ccording to the {OECD}'s {G}lobally {H}armonized {S}ystem of {C}lassification, both extracts are non-toxic orally. {A}ntiplasmodial activity of {T}. macroptera extracts was confirmed in vitro against {P}. falciparum {F}c{B}1 strain with {IC}50 values of 1.2 and 1.6 mu g/m{L} for {TML} and {TMR}, respectively. {I}n vivo, oral administration of {TML} and {TMR} induced significant reduction of parasitaemia (37.2 and 46.4% respectively) in {P}. chabaudi chabaudi infected mice at the 7th day of infection compared to untreated mice. {I}n the cerebral malaria experimental model, mice treated with {TMR} and {TML} presented respectively 50 and 66.7% survival rates at day 9 post-infection when all untreated mice died. {E}leven major compounds were found in {TMR}. {A}mong them, several molecules already known could be responsible for the antiplasmodial activity of the roots extract of {T}. macroptera. {C}onclusions: {T}his study confirms both safety and anti-malarial activity of {T}. macroptera, thus validating its traditional use.},
  keywords = {{A}ntimalarial activity ; {T}erminalia macroptera ; {I}n vitro ; {M}ice models ; {P}lasmodium berghei {ANKA} ; {P}lasmodium chabaudi ; {T}oxicity ; {MALI}},
  booktitle = {},
  journal = {{M}alaria {J}ournal},
  volume = {17},
  numero = {},
  pages = {68 [10 ]},
  ISSN = {1475-2875},
  year = {2018},
  DOI = {10.1186/s12936-018-2223-7},
  URL = {https://www.documentation.ird.fr/hor/fdi:010072341},
}