@article{fdi:010072062,
  title = {{A}pplication of solid phase adsorption toxin tracking ({SPATT}) devices for the field detection of {G}ambierdiscus toxins},
  author = {{R}ou{\'e}, {M}elanie and {D}arius, {H}. {T}. and {V}iallon, {J}. and {U}ng, {A}. and {G}atti, {C}. and {H}arwood, {D}. {T}. and {C}hinain, {M}.},
  editor = {},
  language = {{ENG}},
  abstract = {{C}iguatera fish poisoning is a food-borne illness caused by the consumption of seafood contaminated with ciguatoxins ({CTX}s) produced by dinoflagellates in the {G}ambierdiscus genus. {S}ince most surveillance programs currently rely on the survey of {G}ambierdiscus cell densities and species composition, supplementary toxin-based methods allowing the time- and spatially integrated sampling of toxins in ciguateric environments are needed for a more reliable assessment and management of the risks associated with {G}ambierdiscus proliferation. {S}olid {P}hase {A}dsorption {T}oxin {T}racking ({SPATT}) filters use porous synthetic resins capable of adsorbing toxins directly from the water column. {T}o assess the ability of these passive monitoring devices to retain {G}ambierdiscus toxins, {SPATT} bags filled with 10 g of {HP}20 resin were deployed for 48 h in two {F}rench {P}olynesian locations at high ({N}uku {H}iva {I}sland) vs. low to moderate ({K}aulcura {A}toll) risk of ciguatera. {CTX}s could be detected in {SPATT} bags extracts from {N}uku {H}iva {I}sland, as assessed by the mouse neuroblastoma cell-based assay ({CBA}-{N}2a) and liquid chromatography - tandem mass spectrometry ({LC}-{MS}/{MS}) analyses. {R}esults of in vitro experiments suggest that the saturation limit of {CTX}s on {HP}20 resin, for a deployment time of 48 h, is similar or equal to 55 ng {P}-{CTX}-3{C} equiv. g(-1), resin. {D}espite the non detection of maitotoxin ({MTX}), {LC}-{MS}/{MS} analyses showed that two other compounds also produced by {G}ambierdiscus species were retained on {SPATT} bags, i.e. iso-{P}-{CTX}-3{B}/{C} and a putative {MTX} analogue, known as {MTX}-3. {T}his study, the first to demonstrate the suitability of {SPATT} technology for the in situ monitoring of {G}ambierdiscus toxins, highlights the potential application of this tool for routine ciguatera risk assessment and management programs.},
  keywords = {{SPATT} devices ; {HP}20 resin ; {C}iguatoxins ; {C}iguatera risk monitoring ; {N}euroblastoma cell-based assay ; {LC}-{MS}/{MS} ; {POLYNESIE} {FRANCAISE} ; {PACIFIQUE}},
  booktitle = {},
  journal = {{H}armful {A}lgae},
  volume = {71},
  numero = {},
  pages = {40--49},
  ISSN = {1568-9883},
  year = {2018},
  DOI = {10.1016/j.hal.2017.11.006},
  URL = {https://www.documentation.ird.fr/hor/fdi:010072062},
}