@article{fdi:010072021,
  title = {{A} direct from blood reverse transcriptase polymerase chain reaction assay for monitoring {F}alciparum malaria parasite transmission in elimination settings},
  author = {{T}aylor, {B}. {J}. and {L}anke, {K}. and {B}anman, {S}. {L}. and {M}orlais, {I}sabelle and {M}orin, {M}. {J}. and {B}ousema, {T}. and {R}ijpma, {S}. {R}. and {Y}anow, {S}. {K}.},
  editor = {},
  language = {{ENG}},
  abstract = {{W}e describe a novel one-step reverse transcriptase real-time {PCR} (direct {RT}-{PCR}) for {P}lasmodium falciparum malaria parasites that amplifies {RNA} targets directly from blood. {W}e developed the assay to identify gametocyte-specific transcripts in parasites from patient blood samples, as a means of monitoring malaria parasite transmission in field settings. {T}o perform the test, blood is added directly to a master mix in {PCR} tubes and analyzed by real-time {PCR}. {T}he limit of detection of the assay on both conventional and portable real-time {PCR} instruments was 100 parasites/m{L} for 18{S} r{RNA}, and 1,000 parasites/m{L} for asexual ({PFE}0065{W}) and gametocyte ({PF}14_0367, {PFGEXP}5) m{RNA} targets. {T}he usefulness of this assay in field studies was explored in samples from individuals living in a high-transmission region in {C}ameroon. {T}he sensitivity and specificity of the assay compared with a standard two-step {RT}-{PCR} was 100% for 18{S} r{RNA} on both conventional and portable instruments. {F}or {PF}14_0367, the sensitivity and specificity were 85.7% and 70.0%, respectively, on the conventional instrument and 78.6% and 90%, respectively, on the portable instrument. {T}he concordance for assays run on the two instruments was 100% for 18{S} r{RNA}, and 79.2% for {PF}14_0367, with most discrepancies resulting from samples with low transcript levels. {T}he results show asexual and sexual stage {RNA} targets can be detected directly from blood samples in a simple one-step test on a field-friendly instrument. {T}his assay may be useful for monitoring malaria parasite transmission potential in elimination settings, where sensitive diagnostics are needed to evaluate the progress of malaria eradication initiatives.},
  keywords = {{CAMEROUN}},
  booktitle = {},
  journal = {{A}merican {J}ournal of {T}ropical {M}edicine and {H}ygiene},
  volume = {97},
  numero = {2},
  pages = {533--543},
  ISSN = {0002-9637},
  year = {2017},
  DOI = {10.4269/ajtmh.17-0039},
  URL = {https://www.documentation.ird.fr/hor/fdi:010072021},
}