@article{fdi:010072014,
  title = {{I}mprovement of cowpea productivity by rhizobial and mycorrhizal inoculation in {B}urkina {F}aso},
  author = {{H}aro, {H}. and {S}anon, {K}. {B}. and {L}e {R}oux, {C}. and {D}uponnois, {R}obin and {T}raor{\'e}, {A}. {S}.},
  editor = {},
  language = {{ENG}},
  abstract = {{C}owpea is one of the most important food legume crops in {B}urkina {F}aso. {I}t is able to associate with arbuscular mycorrhizal fungi ({AMF}) and rhizobia. {T}his dual symbiosis improves nitrogen and phosphorus nutrient uptake in cowpea. {A}s the application of exotic inoculants frequently lacks positive responses in field experiments, this study set out to select well-adapted native symbiotic rhizobial and {AMF} strains. {S}oil samples were collected from six study sites in three different climatic zones of {B}urkina {F}aso to investigate their native symbiotic strains. {S}oil-extraction of native spores led to the identification of four {AMF} genera ({S}cutellospora, {G}igaspora, {G}lomus and {E}ntrophospora) by morpho-anatomical characterization. {T}he two most effective cowpea fungal strains were selected after spore isolation from field-collected soils, multiplication on maize roots and inoculation on cowpea seedlings in a greenhouse experiment. {C}owpea-nodulating rhizobial strains were trapped in the greenhouse by planting cowpea seeds in collected soil samples and the strains were characterized using molecular methods. {T}his characterization led to the rhizobial isolates being classified in four clusters on the phylogenetic tree (using the {M}aximum-{L}ikelihood {P}hylogenies method). {A}ll strains belonged to the {B}radyrhizobium genus and most of them were included in the {B}. japonicum branch. {S}ome groups were clearly distinct species already identified and may be new species. {T}he two most effective strains for cowpea yield improvement in the field were selected after cowpea inoculation in a greenhouse experiment. {T}he inoculation design in the field experiment consisted of four single inoculation treatments, either rhizobial or mycorrhizal, along with four dual inoculations, one treatment with chemical fertilizers, and one uninoculated control. {T}he results showed that cowpea productivity was significatively improved by dual inoculation with native rhizobial and mycorrhizal strains, reaching the same level as the application of commonly used chemical fertilizers [{N}itrogen, {P}hosphorus and {P}otassium fertilizers ({NPK})]. {I}n addition, dual inoculation resulted in the highest iron content in cowpea leaves.},
  keywords = {{A}rbuscular mycorrhizal fungi ; {R}hizobia ; {C}owpea ; {M}icrobial inoculation ; {B}urkina {F}aso ; {BURKINA} {FASO}},
  booktitle = {},
  journal = {{S}ymbiosis},
  volume = {74},
  numero = {2},
  pages = {107--120},
  ISSN = {0334-5114},
  year = {2018},
  DOI = {10.1007/s13199-017-0478-3},
  URL = {https://www.documentation.ird.fr/hor/fdi:010072014},
}