Badshah H., Ullah F., Zada H., Zeb Q., Shah R. A., Calatayud Paul-André, Crickmore N. (2017). Comparison of Phenacoccus solenopsis specimens from different regions of Pakistan using COI molecular barcoding (Hemiptera : Pseudococcidae). Annales de la Société Entomologique de France, 53 (6), p. 374-378. ISSN 0037-9271.
Titre du document
Comparison of Phenacoccus solenopsis specimens from different regions of Pakistan using COI molecular barcoding (Hemiptera : Pseudococcidae)
Badshah H., Ullah F., Zada H., Zeb Q., Shah R. A., Calatayud Paul-André, Crickmore N.
Source
Annales de la Société Entomologique de France, 2017,
53 (6), p. 374-378 ISSN 0037-9271
Because correct identification of insects is crucial for pest management involving chemical or biological control agents, we have used a molecular approach to identify and characterize specimens of the cotton pest Phenacoccus solenopsis Tinsley (Sternorrhyncha: Pseudococcidae) present in different regions of Pakistan. The specimens were analyzed through DNA sequence analysis of their mitochondrial COI (mtCOI) gene using an improved procedure that could distinguish between the pest and its associated parasitoid. Our analysis showed no variation among the mealybug specimens from different geographical locations of Pakistan and confirmed that this is the same species and haplotype that is infesting cotton plants in other parts of Asia. This information will assist in the development of biological control programs against P. solenopsis in Pakistan and other Asian countries.
Plan de classement
Sciences fondamentales / Techniques d'analyse et de recherche [020]
;
Sciences du monde végétal [076]
;
Sciences du monde animal [080]
