%0 Journal Article
%9 ACL : Articles dans des revues avec comité de lecture répertoriées par l'AERES
%A Moussavou-Boundzanga, P.
%A Koumakpayi, I. H.
%A Labouba, I.
%A Leroy, Eric
%A Belembaogo, E.
%A Berthet, N.
%T Human papillomavirus detection using the Abbott RealTime high-risk HPV tests compared with conventional nested PCR coupled to high-throughput sequencing of amplification products in cervical smear specimens from a Gabonese female population
%D 2017
%L fdi:010071961
%G ENG
%J Virology Journal
%@ 1743-422X
%K Abbott RealTime high-risk HPV ; Conventional PCR ; Human papillomavirus ; Screening ; Cervical cancer
%K GABON
%M ISI:000418778300001
%P art. 241 [8 ]
%R 10.1186/s12985-017-0906-2
%U https://www.documentation.ird.fr/hor/fdi:010071961
%> https://horizon.documentation.ird.fr/exl-doc/pleins_textes/divers18-01/010071961.pdf
%V 14
%W Horizon (IRD)
%X Background: Cervical cancer is the fourth most common malignancy in women worldwide. However, screening with human papillomavirus (HPV) molecular tests holds promise for reducing cervical cancer incidence and mortality in low-and middle-income countries. The performance of the Abbott RealTime High-Risk HPV test (AbRT) was evaluated in 83 cervical smear specimens and compared with a conventional nested PCR coupled to high-throughput sequencing (HTS) to identify the amplicons. Results: The AbRT assay detected at least one HPV genotype in 44.57% of women regardless of the grade of cervical abnormalities. Except for one case, good concordance was observed for the genotypes detected with the AbRT assay in the high-risk HPV category determined with HTS of the amplicon generated by conventional nested PCR. Conclusions: The AbRT test is an easy and reliable molecular tool and was as sensitive as conventional nested PCR in cervical smear specimens for detection HPVs associated with high-grade lesions. Moreover, sequencing amplicons using an HTS approach effectively identified the genotype of the hrHPV identified with the AbRT test.
%$ 052 ; 050 ; 020