@article{fdi:010071961,
  title = {{H}uman papillomavirus detection using the {A}bbott {R}eal{T}ime high-risk {HPV} tests compared with conventional nested {PCR} coupled to high-throughput sequencing of amplification products in cervical smear specimens from a {G}abonese female population},
  author = {{M}oussavou-{B}oundzanga, {P}. and {K}oumakpayi, {I}. {H}. and {L}abouba, {I}. and {L}eroy, {E}ric and {B}elembaogo, {E}. and {B}erthet, {N}.},
  editor = {},
  language = {{ENG}},
  abstract = {{B}ackground: {C}ervical cancer is the fourth most common malignancy in women worldwide. {H}owever, screening with human papillomavirus ({HPV}) molecular tests holds promise for reducing cervical cancer incidence and mortality in low-and middle-income countries. {T}he performance of the {A}bbott {R}eal{T}ime {H}igh-{R}isk {HPV} test ({A}b{RT}) was evaluated in 83 cervical smear specimens and compared with a conventional nested {PCR} coupled to high-throughput sequencing ({HTS}) to identify the amplicons. {R}esults: {T}he {A}b{RT} assay detected at least one {HPV} genotype in 44.57% of women regardless of the grade of cervical abnormalities. {E}xcept for one case, good concordance was observed for the genotypes detected with the {A}b{RT} assay in the high-risk {HPV} category determined with {HTS} of the amplicon generated by conventional nested {PCR}. {C}onclusions: {T}he {A}b{RT} test is an easy and reliable molecular tool and was as sensitive as conventional nested {PCR} in cervical smear specimens for detection {HPV}s associated with high-grade lesions. {M}oreover, sequencing amplicons using an {HTS} approach effectively identified the genotype of the hr{HPV} identified with the {A}b{RT} test.},
  keywords = {{A}bbott {R}eal{T}ime high-risk {HPV} ; {C}onventional {PCR} ; {H}uman papillomavirus ; {S}creening ; {C}ervical cancer ; {GABON}},
  booktitle = {},
  journal = {{V}irology {J}ournal},
  volume = {14},
  numero = {},
  pages = {art. 241 [8 p.]},
  ISSN = {1743-422{X}},
  year = {2017},
  DOI = {10.1186/s12985-017-0906-2},
  URL = {https://www.documentation.ird.fr/hor/fdi:010071961},
}