@article{fdi:010071959,
  title = {{LUCS} ({L}ight-{U}p {C}ell {S}ystem), a universal high throughput assay for homeostasis evaluation in live cells},
  author = {{D}erick, {S}. and {G}ironde, {C}. and {P}erio, {P}ierre and {R}eybier, {K}. and {N}epveu, {F}. and {J}auneau, {A}. and {F}urger, {C}.},
  editor = {},
  language = {{ENG}},
  abstract = {{O}bservations of fluorescent cyanine dye behavior under illumination at 500 nm lead to a novel concept in cell biology allowing the development of a new live cell assay called {LUCS}, for {L}ight-{U}p {C}ell {S}ystem, measuring homeostasis in live cells. {O}ptimization of the {LUCS} process resulted in a standardized, straightforward and high throughput assay with applications in toxicity assessment. {T}he mechanisms of the {LUCS} process were investigated. {E}lectron {P}aramagnetic {R}esonance experiments showed that the singlet oxygen and hydroxyl radical are involved downstream of the light effect, presumably leading to deleterious oxidative stress that massively opens access of the dye to its intracellular target. {R}eversible modulation of {LUCS} by both verapamil and proton availability indicated that plasma membrane proton/cation antiporters, possibly of the {MATE} drug efflux transport family, are involved. {A} mechanistic model is presented. {O}ur data show that intracellular oxidation can be controlled by tuning light energy, opening applications in regulatory purposes, anti-oxidant research, chemotherapy efficacy and dynamic phototherapy strategies.},
  keywords = {},
  booktitle = {},
  journal = {{S}cientific {R}eports - {N}ature},
  volume = {7},
  numero = {},
  pages = {art. 18069 [ 11 p.]},
  ISSN = {2045-2322},
  year = {2017},
  DOI = {10.1038/s41598-017-18211-2},
  URL = {https://www.documentation.ird.fr/hor/fdi:010071959},
}