Desulfonatronum parangueonense sp. nov., a sulfate-reducing bacterium isolated from sediment of an alkaline crater lake

2017

Article référencé dans le Web of Science WOS:000417933700019

Perez Bernal M. F., Souza Brito E. M., Bartoli M., Aubé J., Fardeau Marie-Laure, Cuevas Rodriguez G., Ollivier Bernard, Guyoneaud R., Hirschler-Rea A.

International Journal of Systematic and Evolutionary Microbiology, 2017, 67 (12), p. 4999-5005 ISSN 1466-5026

Novel Gram-stain-negative, non-spore-forming, vibrio-shaped, anaerobic, alkaliphilic, sulfate-reducing bacteria, designated strains PAR180(T) and PAR190, were isolated from sediments collected at an alkaline crater lake in Guanajuato (Mexico). Strain PAR180(T) grew at temperatures between 15 and 40 degrees C (optimum 35 degrees C), and at pH between 8.3 and 10.4 (optimum 9). It was halotolerant, growing with up to 8% (w/v) NaCl. Lactate, formate, pyruvate and ethanol were used as electron donors in the presence of sulfate and were incompletely oxidized to acetate and CO2. The isolate was able to grow with hydrogen and with CO2 as a carbon source. Beside sulfate, sulfite and thiosulfate were used as terminal electron acceptors. The isolate was able to grow by disproportionation of sulfite and thiosulfate, but not elemental sulfur, using acetate as a carbon source. The predominant fatty acids were C16 : 0, C-16 : (1)omega 7c and summed feature 10 (C-18 : (1)omega 7c and/or C-18 : (1)omega 9t and/or C-18 : (1)omega 12t). The DNA G+C content was 56.1 mol%. Phylogenetic analysis based on 16S rRNA gene sequences showed that it belongs to the genus Desulfonatronum, class Deltaproteobacteria. Its closest relative is Desulfonatronum thiosulfatophilum (98.7% 16S rRNA gene sequence similarity). The DNA-DNA relatedness value between strain PAR180(T) and the type strain of D. thiosulfatophilum was 37.1 +/- 2.5 %. On the basis of phylogenetic, phenotypic and chemotaxonomic characteristics, the isolates is considered to represent a novel species of the genus Desulfonatronum, for which the name Desulfonatronum parangueonense sp. nov. is proposed. The type strain is PAR180(T) (= DSM 103602(T) = JCM 31598(T)).

Limnologie biologique / Océanographie biologique [034] ; Biotechnologies [084]

MEXIQUE

Fonds IRD [F B010071944]

fdi:010071944