@article{fdi:010071872,
  title = {i{DNA} screening : disease vectors as vertebrate samplers},
  author = {{K}ocher, {A}. and de {T}hoisy, {B}. and {C}atzeflis, {F}. and {V}ali{\`e}re, {S}. and {B}anuls, {A}nne-{L}aure and {M}urienne, {J}.},
  editor = {},
  language = {{ENG}},
  abstract = {{I}n the current context of global change and human-induced biodiversity decline, there is an urgent need for developing sampling approaches able to accurately describe the state of biodiversity. {T}raditional surveys of vertebrate fauna involve time-consuming and skill-demanding field methods. {R}ecently, the use of {DNA} derived from invertebrate parasites (leeches and blowflies) was suggested as a new tool for vertebrate diversity assessment. {B}loodmeal analyses of arthropod disease vectors have long been performed to describe their feeding behaviour, for epidemiological purposes. {O}n the other hand, this existing expertise has not yet been applied to investigate vertebrate fauna per se. {H}ere, we evaluate the usefulness of hematophagous dipterans as vertebrate samplers. {B}lood-fed sand flies and mosquitoes were collected in {A}mazonian forest sites and analysed using high-throughput sequencing of short mitochondrial markers. {B}loodmeal identifications highlighted contrasting ecological features and feeding behaviour among dipteran species, which allowed unveiling arboreal and terrestrial mammals of various body size, as well as birds, lizards and amphibians. {A}dditionally, lower vertebrate diversity was found in sites undergoing higher levels of human-induced perturbation. {T}hese results suggest that, in addition to providing precious information on disease vector host use, dipteran bloodmeal analyses may represent a useful tool in the study of vertebrate communities. {A}lthough further effort is required to validate the approach and consider its application to large-scale studies, this first work opens up promising perspectives for biodiversity monitoring and eco-epidemiology.},
  keywords = {bloodmeal ; dipteran ; feeding preference ; insect ; mosquito ; sand fly ; {GUYANE} {FRANCAISE} ; {AMAZONIE}},
  booktitle = {},
  journal = {{M}olecular {E}cology},
  volume = {26},
  numero = {22},
  pages = {6478--6486},
  ISSN = {0962-1083},
  year = {2017},
  DOI = {10.1111/mec.14362},
  URL = {https://www.documentation.ird.fr/hor/fdi:010071872},
}