%0 Journal Article %9 ACL : Articles dans des revues avec comité de lecture répertoriées par l'AERES %A Edouard, S. %A Subramanian, G. %A Lefevre, B. %A Dos Santos, A. %A Pouedras, P. %A Poinsignon, Y. %A Mediannikov, Oleg %A Raoult, Didier %T Co-Infection with Arsenophonus nasoniae and Orientia tsutsugamushi in a traveler %D 2013 %L fdi:010071501 %G ENG %J Vector-Borne and Zoonotic Diseases %@ 1530-3667 %K Arsenophonus nasoniae ; Orientia tsutsugamush ; Co-infection ; Asia ; Traveler %K ASIE DU SUD EST %M ISI:000322967800005 %N 8 %P 565-571 %R 10.1089/vbz.2012.1083 %U https://www.documentation.ird.fr/hor/fdi:010071501 %> https://www.documentation.ird.fr/intranet/publi/depot/2018-03-22/010071501.pdf %V 13 %W Horizon (IRD) %X Here we report a case of co-infection with Orientia tsutsugamushi, the causative agent of scrub typhus, and Arsenophonus nasoniae in a woman with a rash and an eschar who returned from a trip to Southeast Asia. A. nasoniae was previously considered to be a secondary insect and tick endosymbiont of unknown pathogenicity in humans. We amplified both O. tsutsugamushi and A. nasoniae DNA from a skin eschar with qPCR, and a seroconversion for O. tsutsugamushi and A. nasoniae was observed with immunofluorescence assays and western blotting for this patient. And we used 2-D western blotting with an A. nasoniae antigen and polyclonal mouse anti-A. nasoniae antibodies produced in our laboratory to detect the specific antigenic A. nasoniae proteins. %$ 052