@article{fdi:010071497,
  title = {{C}urrent and past strategies for bacterial culture in clinical microbiology},
  author = {{L}agier, {J}. {C}. and {E}douard, {S}. and {P}agnier, {I}. and {M}ediannikov, {O}leg and {D}rancourt, {M}. and {R}aoult, {D}idier},
  editor = {},
  language = {{ENG}},
  abstract = {{A} pure bacterial culture remains essential for the study of its virulence, its antibiotic susceptibility, and its genome sequence in order to facilitate the understanding and treatment of caused diseases. {T}he first culture conditions empirically varied incubation time, nutrients, atmosphere, and temperature; culture was then gradually abandoned in favor of molecular methods. {T}he rebirth of culture in clinical microbiology was prompted by microbiologists specializing in intracellular bacteria. {T}he shell vial procedure allowed the culture of new species of {R}ickettsia. {T}he design of axenic media for growing fastidious bacteria such as {T}ropheryma whipplei and {C}oxiella burnetii and the ability of amoebal coculture to discover new bacteria constituted major advances. {S}trong efforts associating optimized culture media, detection methods, and a microaerophilic atmosphere allowed a dramatic decrease of the time of {M}ycobacterium tuberculosis culture. {T}he use of a new versatile medium allowed an extension of the repertoire of archaea. {F}inally, to optimize the culture of anaerobes in routine bacteriology laboratories, the addition of antioxidants in culture media under an aerobic atmosphere allowed the growth of strictly anaerobic species. {N}evertheless, among usual bacterial pathogens, the development of axenic media for the culture of {T}reponema pallidum or {M}ycobacterium leprae remains an important challenge that the patience and innovations of cultivators will enable them to overcome.},
  keywords = {},
  booktitle = {},
  journal = {{C}linical {M}icrobiology {R}eviews},
  volume = {28},
  numero = {1},
  pages = {208--236},
  ISSN = {0893-8512},
  year = {2015},
  DOI = {10.1128/cmr.00110-14},
  URL = {https://www.documentation.ird.fr/hor/fdi:010071497},
}