Publications des scientifiques de l'IRD

Combes Marie-Christine, Joët Thierry, Lashermes Philippe. (2018). Development of a rapid and efficient DNA-based method to detect and quantify adulterations in coffee (Arabica versus Robusta). Food Control, 88, p. 198-206. ISSN 0956-7135.

Titre du document
Development of a rapid and efficient DNA-based method to detect and quantify adulterations in coffee (Arabica versus Robusta)
Année de publication
2018
Type de document
Article référencé dans le Web of Science WOS:000427343400024
Auteurs
Combes Marie-Christine, Joët Thierry, Lashermes Philippe
Source
Food Control, 2018, 88, p. 198-206 ISSN 0956-7135
Worldwide appreciated beverage, "Arabica" and "Robusta" coffee produced from Coffea arabica and C. canephora respectively, represents a high economical value product. Arabica coffee is generally sold at a higher price than Robusta coffee. As both intentional and accidental adulterations of Arabica with Robusta are frequent, a reliable, inexpensive, and high-throughput adulteration detection technology is required for the coffee industry. We developed a DNA-based authentication method that overcomes limitations of metabolic profiling of Robusta and Arabica coffees. The identification of Arabica and Robusta coffee species, as well as the quantification of their relative proportion in blends were performed by High Resolution Melting (HRM) analysis on green and roasted coffee products. For a more sensitive detection method, chloroplastic rather than nuclear genetic variations were targeted between Coffea species. By the developed method, the Coffea species of origin can be quickly differentiated and in case of adulteration, the percentage can be determined.
Plan de classement
Sciences du monde végétal [076]
Localisation
Fonds IRD [F B010071469]
Identifiant IRD
fdi:010071469
Contact