@article{fdi:010071448,
  title = {{E}nantiomeric fractionation as a tool for quantitative assessment of biodegradation : the case of metoprolol},
  author = {{S}ouchier, {M}. and {B}enali-{R}aclot, {D}. and {C}asellas, {C}. and {I}ngrand, {V}. and {C}hiron, {S}erge},
  editor = {},
  language = {{ENG}},
  abstract = {{A}n efficient chiral liquid chromatography high resolution mass spectrometry method has been developed for the determination of metoprolol ({MTP}) and three of its major metabolites, namely {O}-desmethylmetoprolol ({O}-{DMTP}), alpha-hydroxymetoprolol (alpha-{HMTP}) and metoprolol acid ({MTPA}) in wastewater treatment plant ({WWTP}) influents and effluents. {T}he optimized analytical method has been validated with good quality parameters including resolution >1.3 and method quantification limits down to the ng/{L} range except for {MTPA}. {O}n the basis of this newly developed analytical method, the stereochemistry of {MTP} and its metabolites was studied over time in effluent/sediment biotic and sterile microcosms under dark and light conditions and in influents and effluents of 5 different {WWTP}s. {MTP} stereoselective degradation was exclusively observed under biotic conditions, confirming the specificity of enantiomeric fraction variations to biodegradation processes. {MTP} was always biotransformed into {MTPA} with a ({S})-enantiomer enrichment. {T}he results of enantiomeric enrichment pointed the way for a quantitative assessment of in situ biodegradation processes due to a good fit ({R}-2 > 0.98) of the aerobic {MTP} biodegradation to the {R}ayleigh dependency in all the biotic microcosms and in {WWTP}s because both {MTP} enantiomers followed the same biodegradation kinetic profiles. {T}hese results demonstrate that enantiomeric fractionation constitutes a very interesting quantitative indicator of {MTP} biodegradation in {WWTP}s and probably in the environment.},
  keywords = {{M}etoprolol ; {B}iodegradation ; {E}nantiomeric enrichment ; {R}ayleigh ; {W}astewater treatment},
  booktitle = {},
  journal = {{W}ater {R}esearch},
  volume = {95},
  numero = {},
  pages = {19--26},
  ISSN = {0043-1354},
  year = {2016},
  DOI = {10.1016/j.watres.2016.03.010},
  URL = {https://www.documentation.ird.fr/hor/fdi:010071448},
}