Enantiomeric fractionation as a tool for quantitative assessment of biodegradation : the case of metoprolol

2016

Article référencé dans le Web of Science WOS:000375819000003

Souchier M., Benali-Raclot D., Casellas C., Ingrand V., Chiron Serge

Water Research, 2016, 95, p. 19-26 ISSN 0043-1354

An efficient chiral liquid chromatography high resolution mass spectrometry method has been developed for the determination of metoprolol (MTP) and three of its major metabolites, namely O-desmethylmetoprolol (O-DMTP), alpha-hydroxymetoprolol (alpha-HMTP) and metoprolol acid (MTPA) in wastewater treatment plant (WWTP) influents and effluents. The optimized analytical method has been validated with good quality parameters including resolution >1.3 and method quantification limits down to the ng/L range except for MTPA. On the basis of this newly developed analytical method, the stereochemistry of MTP and its metabolites was studied over time in effluent/sediment biotic and sterile microcosms under dark and light conditions and in influents and effluents of 5 different WWTPs. MTP stereoselective degradation was exclusively observed under biotic conditions, confirming the specificity of enantiomeric fraction variations to biodegradation processes. MTP was always biotransformed into MTPA with a (S)-enantiomer enrichment. The results of enantiomeric enrichment pointed the way for a quantitative assessment of in situ biodegradation processes due to a good fit (R-2 > 0.98) of the aerobic MTP biodegradation to the Rayleigh dependency in all the biotic microcosms and in WWTPs because both MTP enantiomers followed the same biodegradation kinetic profiles. These results demonstrate that enantiomeric fractionation constitutes a very interesting quantitative indicator of MTP biodegradation in WWTPs and probably in the environment.

Sciences fondamentales / Techniques d'analyse et de recherche [020] ; Pollution [038] ; Hydrologie [062]

Fonds IRD [F B010071448]

fdi:010071448