P17, an original host defense peptide from ant venom, promotes antifungal activities of macrophages through the induction of C-type lectin receptors dependent on LTB4-mediated PPAR gamma activation - fdi:010071385 - Horizon

Horizon / Plein textes La base de ressources documentaires de l'IRD

IRD

Publications des scientifiques de l'IRD

Benmoussa K., Authier H., Prat M., AlaEddine M., Lefevre L., Rahabi M. C., Bernad J., Aubouy Agnès, Bonnafe E., Leprince J., Pipy B., Treilhou M., Coste A. (2017). P17, an original host defense peptide from ant venom, promotes antifungal activities of macrophages through the induction of C-type lectin receptors dependent on LTB4-mediated PPAR gamma activation. Frontiers in Immunology, 8, art. 1650 [15 p.]. ISSN 1664-3224

Accès réservé (Intranet IRD) Document en accès réservé (Intranet IRD)

Lien direct chez l'éditeur doi:10.3389/fimmu.2017.01650

Titre
P17, an original host defense peptide from ant venom, promotes antifungal activities of macrophages through the induction of C-type lectin receptors dependent on LTB4-mediated PPAR gamma activation
Année de publication2017
Type de documentArticle référencé dans le Web of Science WOS:000416520500001
AuteursBenmoussa K., Authier H., Prat M., AlaEddine M., Lefevre L., Rahabi M. C., Bernad J., Aubouy Agnès, Bonnafe E., Leprince J., Pipy B., Treilhou M., Coste A.
SourceFrontiers in Immunology, 2017, 8, p. art. 1650 [15 p.]. p. art. 1650 [15 p.] ISSN 1664-3224
RésuméDespite the growing knowledge with regard to the immunomodulatory properties of host defense peptides, their impact on macrophage differentiation and on its associated microbicidal functions is still poorly understood. Here, we demonstrated that the P17, a new cationic antimicrobial peptide from ant venom, induces an alternative phenotype of human monocyte-derived macrophages (h-MDMs). This phenotype is characterized by a C-type lectin receptors (CLRs) signature composed of mannose receptor (MR) and Dectin-1 expression. Concomitantly, this activation is associated to an inflammatory profile characterized by reactive oxygen species (ROS), interleukin (IL)-1 beta, and TNF-alpha release. P17-activated h-MDMs exhibit an improved capacity to recognize and to engulf Candida albicans through the overexpression both of MR and Dectin-1. This upregulation requires arachidonic acid (AA) mobilization and the activation of peroxisome proliferator-activated receptor gamma (PPAR.) nuclear receptor through the leukotriene B4 (LTB4) production. AA/LTB4/PPAR gamma/Dectin-1-MR signaling pathway is crucial for P17-mediated anti-fungal activity of h-MDMs, as indicated by the fact that the activation of this axis by P17 triggered ROS production and inflammasome-dependent IL-1 beta release. Moreover, we showed that the increased anti-fungal immune response of h-MDMs by P17 was dependent on intracellular calcium mobilization triggered by the interaction of P17 with pertussis toxin-sensitive G-protein-coupled receptors on h-MDMs. Finally, we also demonstrated that P17-treated mice infected with C. albicans develop less severe gastrointestinal infection related to a higher efficiency of their macrophages to engulf Candida, to produce ROS and IL-1 beta and to kill the yeasts. Altogether, these results identify P17 as an original activator of the fungicidal response of macrophages that acts upstream PPAR gamma/CLRs axis and offer new immunomodulatory therapeutic perspectives in the field of infectious diseases.
Plan de classementSanté : généralités [050] ; Sciences fondamentales / Techniques d'analyse et de recherche [020]
LocalisationFonds IRD [F B010071385]
Identifiant IRDfdi:010071385
Lien permanenthttp://www.documentation.ird.fr/hor/fdi:010071385

Export des données

Disponibilité des documents

Télechargment fichier PDF téléchargeable

Lien sur le Web lien chez l'éditeur

Accès réservé en accès réservé

HAL en libre accès sur HAL


Accès aux documents originaux :

Le FDI est labellisé CollEx

Accès direct

Bureau du chercheur

Site de la documentation

Espace intranet IST (accès réservé)

Suivi des publications IRD (accès réservé)

Mentions légales

Services Horizon

Poser une question

Consulter l'aide en ligne

Déposer une publication (accès réservé)

S'abonner au flux RSS

Voir les tableaux chronologiques et thématiques

Centres de documentation

Bondy

Montpellier (centre IRD)

Montpellier (MSE)

Nouméa

Papeete

Niamey

Ouagadougou

Tunis

La Paz

Quito