@article{fdi:010071339,
  title = {{V}ariable sensitivity in molecular detection of {Z}ika virus in {E}uropean expert laboratories : external quality assessment, november 2016},
  author = {{C}harrel, {R}. and {M}ogling, {R}. and {P}as, {S}. and {P}apa, {A}. and {B}aronti, {C}{\'e}cile and {K}oopmans, {M}. and {Z}eller, {H}. and {L}eparc-{G}offart, {I}. and {R}eusken, {C}. {B}.},
  editor = {},
  language = {{ENG}},
  abstract = {{Z}ika virus ({ZIKV}) infections are a significant public health concern. {A} strong capability for {ZIKV} detection is an absolute requirement for adequate preparedness and response strategies and individual patient care. {T}he objective of this study was to assess and improve the capability of {E}uropean expert laboratories for molecular testing for {ZIKV} through an external quality assessment ({EQA}) scheme. {L}aboratories were provided a panel of 12 samples which included negative samples, samples containing {A}frican-or {A}sian-lineage {ZIKV} at various concentrations (10(3) to 10(9) copies/ml), and samples containing dengue virus, yellow fever virus, or chikungunya virus. {T}he results were analyzed on the basis of the outcomes of testing for the samples and the extraction and detection method used. {S}amples with a {ZIKV} {RNA} status scored correctly by >50% of the laboratories were designated the core sample. {A} total of 85 panel outcomes were submitted by 50 laboratories in 31 countries. {T}he results designated all samples as core samples. {T}hirty-three percent (28/85) of the panel outcomes identified all samples. {A}nalysis at the laboratory level showed that only 40% of the laboratories (20/50), representing 45% of the countries, scored sufficiently; i.e., they had at least one test operational that scored all core samples correctly. {T}here is a need for improvement of the molecular detection of {ZIKV} in 60% of the participating laboratories. {W}hile the specificity of the tests was more robust, the results of the {EQA} showed large variation in test sensitivity. {I}mprovements should focus on both nucleic acid extraction and {ZIKV} detection methods.},
  keywords = {{EQA} ; {EVD}-{L}ab{N}et ; laboratory response ; {Z}ika virus ; laboratory preparedness ; external quality assessment ; molecular virus diagnostics ; {EUROPE}},
  booktitle = {},
  journal = {{J}ournal of {C}linical {M}icrobiology},
  volume = {55},
  numero = {11},
  pages = {3219--3226},
  ISSN = {0095-1137},
  year = {2017},
  DOI = {10.1128/jcm.00987-17},
  URL = {https://www.documentation.ird.fr/hor/fdi:010071339},
}