@article{fdi:010071326,
  title = {{C}ryopreservation of somatic embryos from {P}etiveria alliacea {L}. by different techniques based on vitrification},
  author = {{P}ettinelli, {J}. {D}. and {S}oares, {B}. {D}. and {C}antelmo, {L}. and {G}arcia, {R}. {D}. and {M}ansur, {E}. and {E}ngelmann, {F}lorent and {G}agliardi, {R}. {F}.},
  editor = {},
  language = {{ENG}},
  abstract = {{P}etiveria alliacea {L}. is a medicinal plant originating from the {A}mazon region. {T}his study describes an efficient cryopreservation protocol for somatic embryos ({SE}s) produced from roots of {P}. alliacea based on the comparison of vitrification, encapsulation-dehydration, and {D} cryo-plate techniques. {W}ith the vitrification technique, {SE}s treated with {PVS}2 solution (0.4 {M} sucrose, 3.3 {M} glycerol, 2.4 {M} ethylene glycol, and 1.9 {M} {DMSO}) for 30 min displayed high viability (85%) and intermediate proliferation recovery (about 12 adventitious {SE}s produced from original {SE}s [{SE}s/{SE}] after 90 d of culture). {W}ith the encapsulation-dehydration technique, lower viability (70%) and very low proliferation recovery (about two {SE}s/{SE}) were achieved with cryopreserved {SE}s dehydrated for 10 min in a laminar air flow cabinet. {T}he {D} cryo-plate technique led to high viability (85%) and proliferation recovery (19 {SE}s/{SE}) of cryopreserved {SE}s after 90 min dehydration. {I}n the experimental conditions tested, the {D} cryo-plate method was the most efficient technique for cryopreservation of {P}. alliacea {SE}s.},
  keywords = {{G}uinea ; {C}ryostorage ; {E}ncapsulation ; {D}ehydration ; {D} cryo-plate ; {GUINEE}},
  booktitle = {},
  journal = {{I}n {V}itro {C}ellular and {D}evelopmental {B}iology : {P}lant},
  volume = {53},
  numero = {4},
  pages = {339--345},
  ISSN = {1054-5476},
  year = {2017},
  DOI = {10.1007/s11627-017-9820-y},
  URL = {https://www.documentation.ird.fr/hor/fdi:010071326},
}