Pettinelli J. D., Soares B. D., Cantelmo L., Garcia R. D., Mansur E., Engelmann Florent, Gagliardi R. F. (2017). Cryopreservation of somatic embryos from Petiveria alliacea L. by different techniques based on vitrification. In Vitro Cellular and Developmental Biology : Plant, 53 (4), p. 339-345. ISSN 1054-5476.
Titre du document
Cryopreservation of somatic embryos from Petiveria alliacea L. by different techniques based on vitrification
Pettinelli J. D., Soares B. D., Cantelmo L., Garcia R. D., Mansur E., Engelmann Florent, Gagliardi R. F.
Source
In Vitro Cellular and Developmental Biology : Plant, 2017,
53 (4), p. 339-345 ISSN 1054-5476
Petiveria alliacea L. is a medicinal plant originating from the Amazon region. This study describes an efficient cryopreservation protocol for somatic embryos (SEs) produced from roots of P. alliacea based on the comparison of vitrification, encapsulation-dehydration, and D cryo-plate techniques. With the vitrification technique, SEs treated with PVS2 solution (0.4 M sucrose, 3.3 M glycerol, 2.4 M ethylene glycol, and 1.9 M DMSO) for 30 min displayed high viability (85%) and intermediate proliferation recovery (about 12 adventitious SEs produced from original SEs [SEs/SE] after 90 d of culture). With the encapsulation-dehydration technique, lower viability (70%) and very low proliferation recovery (about two SEs/SE) were achieved with cryopreserved SEs dehydrated for 10 min in a laminar air flow cabinet. The D cryo-plate technique led to high viability (85%) and proliferation recovery (19 SEs/SE) of cryopreserved SEs after 90 min dehydration. In the experimental conditions tested, the D cryo-plate method was the most efficient technique for cryopreservation of P. alliacea SEs.
Plan de classement
Sciences du monde végétal [076]
;
Biotechnologies [084]
