%0 Journal Article
%9 ACL : Articles dans des revues avec comité de lecture répertoriées par l'AERES
%A Ehrlich, H.
%A Bazhenov, V. V.
%A Debitus, Cécile
%A de Voogd, N.
%A Galli, R.
%A Tsurkan, M. V.
%A Wysokowski, M.
%A Meissner, H.
%A Bulut, E.
%A Kaya, M.
%A Jesionowski, T.
%T Isolation and identification of chitin from heavy mineralized skeleton of Suberea clavata (Verongida : Demospongiae : Porifera) marine demosponge
%B Chemistry, environmental, biotechnology and biomedical aspects of chitin and chitosan : APCCS 2016
%D 2017
%E Jayakumar, R
%E Meenakshi, S.
%L fdi:010071275
%G ENG
%J International Journal of Biological Macromolecules
%@ 0141-8130
%K Chitin ; sponges ; Suberea clavata
%K INDONESIE ; BALI
%M ISI:000412959500037
%N Part B (no spécial)
%P 1706-1712
%R 10.1016/j.ijbiomac.2017.01.141
%U https://www.documentation.ird.fr/hor/fdi:010071275
%> https://www.documentation.ird.fr/intranet/publi/2017/11/010071275.pdf
%V 104
%W Horizon (IRD)
%X Since the discovery of chitin in skeletal structures of sponges (Porifera) in 2007, studies on search of novel species which possess this structural aminopolysaccharide continue up today. The most potential source of chitin is suggested to be localized in the four families of sponges related to the order Verongida (Demospongiae) which nevertheless require further clarification. Here, we report for the first time the isolation and identification of alpha-chitin from the Suberea clavata demosponge (Aplysinidae: Verongida). Raman spectroscopy, Calcofluor White staining, chitinase test and ESI-MS techniques were used to identify chitin. We suggest that the presence of chitin within fibrous skeletons of diverse species of Verongida order, and, especially in all species of the Aplysinidae family, may be useful for the identification of novel, previously unidentified marine demosponges.
%B APCCS 2016 : Chemistry, Environmental, Biotechnology and Biomedical Aspects of Chitin and Chitosan
%$ 034 ; 020