@article{fdi:010071031,
  title = {{T}ype 3 {S}ecretion {S}ystem ({T}3{SS}) of {B}radyrhizobium sp {DOA}9 and its roles in legume symbiosis and rice endophytic association},
  author = {{S}ongwattana, {P}. and {N}oisangiam, {R}. and {T}eamtisong, {K}. and {P}rakamhang, {J}. and {T}eulet, {A}. and {T}ittabutr, {P}. and {P}iromyou, {P}. and {B}oonkerd, {N}. and {G}iraud, {E}ric and {T}eaumroong, {N}.},
  editor = {},
  language = {{ENG}},
  abstract = {{T}he {B}radyrhizobium sp. {DOA}9 strain isolated from a paddy field has the ability to nodulate a wide spectrum of legumes. {U}nlike other bradyrhizobia, this strain has a symbiotic plasmid harboring nod, nif, and type 3 secretion system ({T}3{SS}) genes. {T}his {T}3{SS} cluster contains all the genes necessary for the formation of the secretory apparatus and the transcriptional activator ({T}ts{I}), which is preceded by a nod-box motif. {A}n in silico search predicted 14 effectors putatively translocated by this {T}3{SS} machinery. {I}n this study, we explored the role of the {T}3{SS} in the symbiotic performance of {DOA}9 by evaluating the ability of a {T}3{SS} mutant ({O}mega rhc{N}) to nodulate legumes belonging to {D}albergioid, {M}illettioid, and {G}enistoid tribes. {A}mong the nine species tested, four ({A}rachis hypogea, {V}igna radiata, {C}rotalaria juncea, and {M}acroptilium atropurpureum) responded positively to the rhc{N} mutation (ranging from suppression of plant defense reactions, an increase in the number of nodules and a dramatic improvement in nodule development and infection), one ({S}tylosanthes hamata) responded negatively (fewer nodules and less nitrogen fixation) and four species ({A}eschynomene americana, {A}eschynomene afraspera, {I}ndigofera tinctoria, and {D}esmodium tortuosum) displayed no phenotype. {W}e also tested the role of the {T}3{SS} in the ability of the {DOA}9 strain to endophytically colonize rice roots, but detected no effect of the {T}3{SS} mutation, in contrast to what was previously reported in the {B}radyrhizobium {SUTN}9-2 strain. {T}aken together, these data indicate that {DOA}9 contains a functional {T}3{SS} that interferes with the ability of the strain to interact symbiotically with legumes but not with rice.},
  keywords = {type 3 secretion system ; effector proteins ; {B}radyrhizobium ; legume ; symbiosis},
  booktitle = {},
  journal = {{F}rontiers in {M}icrobiology},
  volume = {8},
  numero = {},
  pages = {art. 1810 [12 p.]},
  ISSN = {1664-302{X}},
  year = {2017},
  DOI = {10.3389/fmicb.2017.01810},
  URL = {https://www.documentation.ird.fr/hor/fdi:010071031},
}