@article{fdi:010071027,
  title = {{T}he importance of simulated lung fluid ({SLF}) extractions for a more relevant evaluation of the oxidative potential of particulate matter},
  author = {{C}alas, {A}. and {U}zu, {G}a{\¨e}lle and {M}artins, {J}. {M}. {F}. and {V}oisin, {D}. and {S}padini, {L}. and {L}acroix, {T}. and {J}affrezo, {J}. {L}.},
  editor = {},
  language = {{ENG}},
  abstract = {{P}articulate matter ({PM}) induces oxidative stress in vivo, leading to adverse health effects. {O}xidative potential ({OP}) of {PM} is increasingly studied as a relevant metric for health impact (instead of {PM} mass concentration) as much of the ambient particle mass do not contribute to {PM} toxicity. {S}everal assays have been developed to quantify {PM} oxidative potential and a widely used one is the acellular dithiothreitol ({DTT}) assay. {H}owever in such assays, particles are usually extracted with methanol or {M}illi-{Q} water which is unrepresentative of physiological conditions. {F}or this purpose, {OPDTT} measurements after simulated lung fluids ({SLF}) extraction, in order to look at the impact of simulated lung fluid constituents, were compared to {M}illi-{Q} water extraction measurements. {O}ur major finding is a significant decrease of the {OPDTT} when the artificial lysosomal fluid ({ALF}) solution was used. {I}ndeed, ligand compounds are present in the {SLF} solutions and some induce a decrease of the {OP} when compared to water extraction. {O}ur results suggest that the effect of ligands and complexation in lining fluids towards {PM} contaminants probably has been underestimated and should be investigated further.},
  keywords = {},
  booktitle = {},
  journal = {{S}cientific {R}eports - {N}ature},
  volume = {7},
  numero = {},
  pages = {art. 11617 [12 p.]},
  ISSN = {2045-2322},
  year = {2017},
  DOI = {10.1038/s41598-017-11979-3},
  URL = {https://www.documentation.ird.fr/hor/fdi:010071027},
}