@article{fdi:010071025,
  title = {{C}o-{I}nfection of mosquitoes with chikungunya and dengue viruses reveals modulation of the replication of both viruses in midguts and salivary glands of {A}edes aegypti mosquitoes},
  author = {{L}e {C}oupanec, {A}. and {T}chankouo-{N}guetcheu, {S}. and {R}oux, {P}. and {K}hun, {H}. and {H}uerre, {M}. and {M}orales-{V}argas, {R}. and {E}nguehard, {M}. and {L}avillette, {D}. and {M}iss{\'e}, {D}oroth{\'e}e and {C}houmet, {V}.},
  editor = {},
  language = {{ENG}},
  abstract = {{A}rthropod-borne virus (arbovirus) infections cause several emerging and resurgent infectious diseases in humans and animals. {C}hikungunya-affected areas often overlap with dengue-endemic areas. {C}oncurrent dengue virus ({DENV}) and chikungunya virus ({CHIKV}) infections have been detected in travelers returning from regions of endemicity. {CHIKV} and {DENV} co-infected {A}edes albopictus have also been collected in the vicinity of co-infected human cases, emphasizing the need to study co-infections in mosquitoes. {W}e thus aimed to study the pathogen-pathogen interaction involved in these co-infections in {DENV}/{CHIKV} co-infected {A}edes aegypti mosquitoes. {I}n mono-infections, we detected {CHIKV} antigens as early as 4 days post-virus exposure in both the midgut ({MG}) and salivary gland ({SG}), whereas we detected {DENV} serotype 2 ({DENV}-2) antigens from day 5 post-virus exposure in {MG} and day 10 post-virus exposure in {SG}. {I}dentical infection rates were observed for singly and co-infected mosquitoes, and facilitation of the replication of both viruses at various times post-viral exposure. {W}e observed a higher replication for {DENV}-2 in {SG} of co-infected mosquitoes. {W}e showed that mixed {CHIKV} and {DENV} infection facilitated viral replication in {A}e. aegypti. {T}he outcome of these mixed infections must be further studied to increase our understanding of pathogen-pathogen interactions in host cells.},
  keywords = {{A}edes aegypti ; arbovirus ; chikungunya ; dengue ; co-infection},
  booktitle = {},
  journal = {{I}nternational {J}ournal of {M}olecular {S}ciences},
  volume = {18},
  numero = {8},
  pages = {art. 1708 [17 p.]},
  ISSN = {1422-0067},
  year = {2017},
  DOI = {10.3390/ijms18081708},
  URL = {https://www.documentation.ird.fr/hor/fdi:010071025},
}