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Marynowska M., Goux X., Sillam Dusses David, Rouland Lefèvre Corinne, Roisin Y., Delfosse P., Calusinska M. (2017). Optimization of a metatranscriptomic approach to study the lignocellulolytic potential of the higher termite gut microbiome. BMC Genomics, 18, art. 681 [ 14 p.]. ISSN 1471-2164

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Lien direct chez l'éditeur doi:10.1186/s12864-017-4076-9

Titre
Optimization of a metatranscriptomic approach to study the lignocellulolytic potential of the higher termite gut microbiome
Année de publication2017
Type de documentArticle référencé dans le Web of Science WOS:000408862200001
AuteursMarynowska M., Goux X., Sillam Dusses David, Rouland Lefèvre Corinne, Roisin Y., Delfosse P., Calusinska M.
SourceBMC Genomics, 2017, 18, p. art. 681 [ 14 p.]. p. art. 681 [ 14 p.] ISSN 1471-2164
RésuméBackground: Thanks to specific adaptations developed over millions of years, the efficiency of lignin, cellulose and hemicellulose decomposition of higher termite symbiotic system exceeds that of many other lignocellulose utilizing environments. Especially, the examination of its symbiotic microbes should reveal interesting carbohydrate-active enzymes, which are of primary interest for the industry. Previous metatranscriptomic reports (high-throughput mRNA sequencing) highlight the high representation and overexpression of cellulose and hemicelluloses degrading genes in the termite hindgut digestomes, indicating the potential of this technology in search for new enzymes. Nevertheless, several factors associated with the material sampling and library preparation steps make the metatranscriptomic studies of termite gut prokaryotic symbionts challenging. Methods: In this study, we first examined the influence of the sampling strategy, including the whole termite gut and luminal fluid, on the diversity and the metatranscriptomic profiles of the higher termite gut symbiotic bacteria. Secondly, we evaluated different commercially available kits combined in two library preparative pipelines for the best bacterial mRNA enrichment strategy. Results: We showed that the sampling strategy did not significantly impact the generated results, both in terms of the representation of the microbes and their transcriptomic profiles. Nevertheless collecting luminal fluid reduces the co-amplification of unwanted RNA species of host origin. Furthermore, for the four studied higher termite species, the library preparative pipeline employing Ribo-Zero Gold rRNA Removal Kit "Epidemiology" in combination with Poly(A) Purist MAG kit resulted in a more efficient rRNA and poly-A-mRNAdepletion (up to 98.44% rRNA removed) than the pipeline utilizing MICROBExpress and MICROBEnrich kits. High correlation of both Ribo-Zero and MICROBExpresse depleted gene expression profiles with total non-depleted RNA-seq data has been shown for all studied samples, indicating no systematic skewing of the studied pipelines. Conclusions: We have extensively evaluated the impact of the sampling strategy and library preparation steps on the metatranscriptomic profiles of the higher termite gut symbiotic bacteria. The presented methodological approach has great potential to enhance metatranscriptomic studies of the higher termite intestinal flora and to unravel novel carbohydrate-active enzymes.
Plan de classementBiologie du sol [074] ; Biotechnologies [084]
Descr. géo.GUYANE FRANCAISE
LocalisationFonds IRD [F B010070955]
Identifiant IRDfdi:010070955
Lien permanenthttp://www.documentation.ird.fr/hor/fdi:010070955

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